三核苷酸在293K和77K时的本征发射特性。

Intrinsic emission properties of trinucleotides at 293 and 77 K.

作者信息

Imakubo K

出版信息

Nucleic Acids Symp Ser. 1985(16):17-20.

Abstract

The intrinsic emission properties of two pairs of isomeric trinucleotides, ApCpGp-CpApGp and ApUpGp-UpApGp, in neutral pH solvent between 293 and 77 K were studied. On the basis of the results obtained upon their emission spectroscopy, the fluorescence of these trinucleotides both at 293 and 77K can be regarded as the intramolecular excimer fluorescence which is red-shifted and broadened compared with that of corresponding equimolar mixture of constituent mononucleotides. On the other hand, the phosphorescence of two pairs of isomers observed at 77K is a characteristic of adenine. Thus, it is assumed that in above trinucleotides, the electronic excitation energy transfer takes place via the excimer state to the lowest triplet state of adenine.

摘要

研究了两对异构三核苷酸ApCpGp-CpApGp和ApUpGp-UpApGp在293至77K的中性pH溶剂中的固有发射特性。根据它们发射光谱的结果，这些三核苷酸在293K和77K时的荧光都可视为分子内激基缔合物荧光，与相应等摩尔组成单核苷酸混合物相比，其发生了红移并变宽。另一方面，在77K观察到的两对异构体的磷光是腺嘌呤的特征。因此，推测在上述三核苷酸中，电子激发能通过激基缔合物状态转移至腺嘌呤的最低三重态。