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超螺旋质粒DNA中碱基臭氧降解的序列特异性

Sequence specificity of ozone-degradation of bases in supercoiled plasmid DNA.

作者信息

Sawadaishi K, Miura K, Ohtsuka E, Ueda T, Ishizaki K, Shinriki N

出版信息

Nucleic Acids Symp Ser. 1985(16):205-8.

PMID:4088871
Abstract

It was found that ozone reacted preferentially with thymine and guanine residues located in the specific region in pBR322 DNA. The sequence analysis of the region including the cleavage site produced by ozonization of ccDNA showed that ozone-modification proceeded in the single stranded region formed by cruciform-formation in supercoiled DNA.

摘要

研究发现,臭氧优先与pBR322 DNA特定区域中的胸腺嘧啶和鸟嘌呤残基发生反应。对包括cccDNA臭氧氧化产生的切割位点在内的区域进行序列分析表明,臭氧修饰发生在超螺旋DNA中十字形结构形成的单链区域。

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