质粒DNA被臭氧降解的模式。
Mode of degradation of plasmid DNA with ozone.
作者信息
Sawadaishi K, Miura K, Ohthuka E, Ueda T, Ishizaki K, Shinriki N
出版信息
Nucleic Acids Symp Ser. 1984(15):151-4.
PMID:6097879
Abstract
The ozonization of pBR322 closed circular DNA showed the conversion to open circular DNA. The damaged site was investigated by restriction mapping. The results showed the damage and subsequent cleavage of the DNA strand of ccDNA by ozonization may occur at the region sensitive to nuclease S1.
摘要
pBR322闭环DNA的臭氧处理显示其转化为开环DNA。通过限制性酶切图谱研究了损伤位点。结果表明，ccDNA的DNA链经臭氧处理后的损伤及随后的切割可能发生在对核酸酶S1敏感的区域。
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