Wang Jieh-Neng, Tu Zih-Yu, Wang Wen-Jyun, Li Wei-Peng, Chen Wei-Ling, Kan Chung-Dann
Department of Pediatrics, College of Medicine, National Cheng Kung University Hospital, Tainan 704, Taiwan.
Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
ACS Appl Bio Mater. 2025 Sep 15;8(9):7616-7627. doi: 10.1021/acsabm.5c00135. Epub 2025 Sep 1.
Medical gases, particularly oxygen and nitric oxide (NO), have attracted significant interest in clinical applications, notably wound healing, due to their role in enhancing cell proliferation, angiogenesis, and collagen deposition. However, the use of these gases has been limited by challenges such as inefficient gas delivery and potential toxicity to normal tissues. In this study, we elucidate a feasible approach using a porphyrin-based metal-organic framework (MOF), a unique material that shows immense potential for dual-gas-assisted wound healing. The MOF nanorods, meticulously designed, contain catalytically active manganese clusters, enabling spontaneous water decomposition and subsequent oxygen generation upon water exposure. The MOF incorporates Fe-chelated porphyrins, which not only serve as ligands connecting the manganese clusters but also exhibit a strong affinity with NO gas for the successful delivery of NO. The loaded NO, tethered to the MOF, can be released in a water environment. We employed a wound-healing assay to evaluate the efficacy of the NO-loaded MOF. After adding the MOF to fibroblast cell culture for O and NO supply, significantly accelerated migration and proliferation were obtained, providing strong evidence for the potential of the MOF in water-driven dual-gas therapy for wound care.
医用气体，特别是氧气和一氧化氮（NO），因其在促进细胞增殖、血管生成和胶原蛋白沉积方面的作用，在临床应用中，尤其是伤口愈合方面引起了广泛关注。然而，这些气体的使用受到了诸如气体输送效率低下以及对正常组织潜在毒性等挑战的限制。在本研究中，我们阐明了一种使用基于卟啉的金属有机框架（MOF）的可行方法，这种独特的材料在双气辅助伤口愈合方面显示出巨大潜力。精心设计的MOF纳米棒含有具有催化活性的锰簇，在接触水时能够自发分解水并随后产生氧气。该MOF包含铁螯合卟啉，其不仅作为连接锰簇的配体，而且与NO气体具有很强的亲和力，从而成功实现NO的输送。负载在MOF上的NO可以在水环境中释放。我们采用伤口愈合试验来评估负载NO的MOF的功效。将MOF添加到成纤维细胞培养物中以提供氧气和NO后，细胞迁移和增殖显著加速，这为MOF在水驱动双气疗法治疗伤口护理方面的潜力提供了有力证据。
