Ramoji Anuradha, Baumbach Philipp, Ryabchykov Oleg, Pistiki Aikaterini, Rueger Jan, Pinzon David Vasquez, Silge Anja, Deinhardt-Emmer Stefanie, Schie Iwan W, Weber Karina, Neu Charles, Neugebauer Ute, Kiehntopf Michael, Bocklitz Thomas, Popp Juergen, Coldewey Sina M
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany.
Leibniz Institute of Photonic Technology - Member of the Research Alliance "Leibniz Health Technologies", Member of the Leibniz Centre For Photonics in Infection Research (LPI), Jena, Germany.
Biotechnol J. 2025 Sep;20(9):e70105. doi: 10.1002/biot.70105.
Sepsis remains a major clinical challenge, often resulting in long-term physiological and immunological disturbances. This study employed high-throughput single-cell Raman spectroscopy to analyze the biochemical profiles of peripheral blood leukocytes from patients with non-COVID-19 and COVID-19-associated sepsis. Leukocytes were assessed at multiple timepoints, including the acute phase (Days 3 and 7 after sepsis onset) and late recovery phase (6 and 12 months after sepsis onset). Raman spectroscopic profiles of leukocytes showed clear separation between healthy controls and sepsis patients during the acute phase with high balanced accuracy (BAcc: 95%-98%). Spectral differences between acute and recovery phases (BAcc: 84%-97%) and between recovery-phase leukocytes and those from healthy controls (BAcc: 81%-90%) were also observed, indicating long-lasting molecular alterations. Furthermore, distinct profiles were identified between non-COVID-19 and COVID-19-associated sepsis during the acute phase (BAcc: 65%-71%) and in the late-recovery phase (BAcc: 71%-83%). These findings demonstrate that Raman spectroscopy enables label-free, high-throughput profiling of leukocyte biochemistry across the sepsis trajectory. This suggests that Raman spectroscopy is a promising tool for high-throughput screening, offering insights into the biomolecular changes in sepsis and providing a diagnostic platform to differentiate between sepsis etiologies, a significant advancement in the field of sepsis diagnostics.
脓毒症仍然是一个重大的临床挑战，常常导致长期的生理和免疫紊乱。本研究采用高通量单细胞拉曼光谱技术，分析非新冠病毒感染和新冠病毒感染相关脓毒症患者外周血白细胞的生化特征。在多个时间点对白细胞进行评估，包括急性期（脓毒症发作后第3天和第7天）和恢复后期（脓毒症发作后6个月和12个月）。白细胞的拉曼光谱特征显示，在急性期，健康对照与脓毒症患者之间有明显区分，平衡准确率较高（BAcc：95%-98%）。还观察到急性期与恢复期之间（BAcc：84%-97%）以及恢复期白细胞与健康对照白细胞之间（BAcc：81%-90%）的光谱差异，表明存在长期的分子改变。此外，在急性期（BAcc：65%-71%）和恢复后期（BAcc：71%-83%），非新冠病毒感染和新冠病毒感染相关脓毒症之间也发现了不同的特征。这些发现表明，拉曼光谱能够对脓毒症病程中的白细胞生物化学进行无标记、高通量分析。这表明拉曼光谱是一种有前景的高通量筛查工具，能够深入了解脓毒症中的生物分子变化，并提供一个区分脓毒症病因的诊断平台，这是脓毒症诊断领域的一项重大进展。
