Su Laijin, Yang Ying, Xu Yangli, Zhu Lianlian, Sun Bolun, Zhou Jiali
College of Life and Environmental Science, Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou 325035, China.
Wenzhou Academy of Agricultural Science, Wenzhou 325006, China.
Food Funct. 2025 Sep 2. doi: 10.1039/d5fo02879e.
This study investigated the digestion characteristics of the polysaccharide fermented using (LA-rSFP) and its effects on dextran sulfate sodium-induced intestinal inflammation in zebrafish. The treatment substantially decreased the molecular weight of LA-rSFP and increased the reducing sugar content during gastrointestinal digestion. LA-rSFP considerably enhanced the activities of superoxide dismutase and catalase and markedly decreased malondialdehyde content, indicating a reduction in oxidative stress in the intestinal environment. Moreover, LA-rSFP ameliorated colitis-induced intestinal injury and upregulated the expression of tight junction proteins (occludin, ZO-1, and claudin-1), suggesting strengthened intestinal barrier integrity. LA-rSFP also played an important role in regulating pro-inflammatory cytokines (downregulated) and anti-inflammatory cytokines (upregulated) during inflammatory responses in the zebrafish gut. The results of 16S rRNA sequencing showed that LA-rSFP increased the diversity and richness of the intestinal microbiota in zebrafish with inflammatory bowel disease (IBD), enhancing the abundance of beneficial bacteria, such as , , , and , and reducing levels. These findings suggest that the administration of LA-rSFP alleviates IBD, with the therapeutic mechanisms partially attributed to the regulation of the intestinal microbiota composition and abundance.
本研究调查了用（LA-rSFP）发酵的多糖的消化特性及其对硫酸葡聚糖钠诱导的斑马鱼肠道炎症的影响。在胃肠道消化过程中，该处理显著降低了LA-rSFP的分子量并增加了还原糖含量。LA-rSFP显著增强了超氧化物歧化酶和过氧化氢酶的活性，并显著降低了丙二醛含量，表明肠道环境中的氧化应激有所减轻。此外，LA-rSFP改善了结肠炎诱导的肠道损伤，并上调了紧密连接蛋白（闭合蛋白、ZO-1和claudin-1）的表达，表明肠道屏障完整性得到加强。LA-rSFP在斑马鱼肠道炎症反应过程中对促炎细胞因子（下调）和抗炎细胞因子（上调）的调节中也发挥了重要作用。16S rRNA测序结果表明，LA-rSFP增加了患有炎症性肠病（IBD）的斑马鱼肠道微生物群的多样性和丰富度，提高了有益细菌（如 、 、 和 ）的丰度，并降低了 水平。这些发现表明，给予LA-rSFP可缓解IBD，其治疗机制部分归因于对肠道微生物群组成和丰度的调节。
