Chun Sang-Young, Park Eun-Hye, Jang Mari, Park Jae-Il, Jang You-Jee
School of Biological Sciences & Technology, Faculty of Life Science, Chonnam National University, Gwangju, Republic of Korea.
Department of Biomedical Laboratory Science, Honam University, Gwangju, Republic of Korea.
Reproduction. 2025 Sep 19;170(4). doi: 10.1530/REP-25-0066. Print 2025 Oct 1.
Sulfiredoxin (Srxn1) is essential for corpus luteum formation during ovulation. Inhibition of Srxn1 with J14 suppressed LH-stimulated progesterone production, key gene expressions (Cyp11a1, Star), and markers of luteinization. This highlights Srxn1's role in promoting LH-induced luteinization through the ERK, C/EBPβ, and Cyp11a1 pathways.
Sulfiredoxin (Srxn1), an antioxidant enzyme, is expressed during ovulation. This study aimed to investigate the physiological function of Srxn1 in corpus luteum formation during the ovulatory process in rats. Treatment of cultured preovulatory follicles with the Srxn1 inhibitor, J14, suppressed LH-stimulated progesterone production, but not estradiol production, in a dose-dependent manner. Likewise, LH-stimulated gene expression of Cyp11a1 and Star, but not Cyp19a1, was suppressed by J14. Regulation of Cyp11a1 and STAR expression by Srxn1 was confirmed using si-Srxn1 in KGN cells, a human granulosa cell line. Furthermore, treatment of preovulatory granulosa cells with J14 dose-dependently suppressed LH-stimulated mRNA and protein levels of C/EBPβ and ERK1/2. Finally, hypertrophy, lipid droplets, progesterone production, and p27Kip1 expression stimulated by LH were significantly lowered by J14 in in vitro luteinization of granulosa cells. Taken together, the present data indicate that Srxn1 plays a significant role in the formation of the corpus luteum during ovulation by stimulating the pathways of ERK, C/EBPβ, and Cyp11a1.
硫氧还蛋白（Srxn1）在排卵期间的黄体形成过程中至关重要。用J14抑制Srxn1可抑制促黄体生成素（LH）刺激的孕酮生成、关键基因表达（Cyp11a1、Star）以及黄体化标志物。这突出了Srxn1在通过细胞外信号调节激酶（ERK）、C/EBPβ和Cyp11a1途径促进LH诱导的黄体化中的作用。
硫氧还蛋白（Srxn1）是一种抗氧化酶，在排卵期间表达。本研究旨在探讨Srxn1在大鼠排卵过程中黄体形成的生理功能。用Srxn1抑制剂J14处理培养的排卵前卵泡，以剂量依赖的方式抑制了LH刺激的孕酮生成，但不影响雌二醇生成。同样，J14抑制了LH刺激的Cyp11a1和Star基因表达，但不影响Cyp19a1的表达。在人颗粒细胞系KGN细胞中使用小干扰RNA（si - Srxn1）证实了Srxn1对Cyp11a1和STAR表达的调节作用。此外，用J14处理排卵前颗粒细胞可剂量依赖地抑制LH刺激的C/EBPβ和ERK1/2的mRNA和蛋白水平。最后，在颗粒细胞的体外黄体化过程中，J14显著降低了LH刺激的肥大、脂滴、孕酮生成和p27Kip1表达。综上所述，目前的数据表明Srxn1通过刺激ERK、C/EBPβ和Cyp11a1途径在排卵期间的黄体形成中发挥重要作用。
