Seo Jewoo, Kim Seung Ha, Lee Jaegeon, Kim Min Seok, Lee Yong-Seok, Kim Sang Jeong
Department of Physiology, Seoul National University College of Medicine, Seoul, Republic of Korea.
Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea.
Elife. 2025 Sep 2;13:RP95838. doi: 10.7554/eLife.95838.
Climbing fiber (CF) transmission from the inferior olive (IO) triggers complex spikes (Cs) in Purkinje cells (PCs) driven by a burst of calcium spikes. In the context of motor learning, especially the compensatory optic response, CF transmission serves as an instructive signal selectively conveyed to PCs. While the significance of CF input in motor memory formation is widely acknowledged, a comprehensive understanding of its distinct contribution across different temporal windows, spanning from the initial learning phase to the retrieval period, remains incomplete. Therefore, we aimed to investigate the necessity of CF-induced instructive signals in motor learning by assessing their roles in memory acquisition, consolidation, and retrieval. We employed optogenetics to selectively inhibit CF transmission during targeted time windows. Consequently, the absence of CF-induced instructive signals during motor learning impairs memory acquisition. However, when these signals were suppressed during the consolidation and retrieval period, there was neither a loss of long-term memory nor a prevention of memory retrieval. Our results highlight that CF transmission plays a specialized and critical role primarily in memory acquisition, rather than in subsequent processes.
来自下橄榄核（IO）的攀缘纤维（CF）传递由一阵钙峰驱动，触发浦肯野细胞（PC）中的复合峰（Cs）。在运动学习的背景下，尤其是代偿性视觉反应，CF传递作为一种有指导意义的信号选择性地传递给PC。虽然CF输入在运动记忆形成中的重要性已得到广泛认可，但对于其在从初始学习阶段到检索期的不同时间窗口中的独特贡献，仍缺乏全面的了解。因此，我们旨在通过评估CF诱导的指导信号在记忆获取、巩固和检索中的作用，来研究其在运动学习中的必要性。我们采用光遗传学方法在特定的时间窗口内选择性抑制CF传递。结果表明，运动学习过程中缺乏CF诱导的指导信号会损害记忆获取。然而，当这些信号在巩固和检索期被抑制时，既不会导致长期记忆丧失，也不会阻止记忆检索。我们的结果表明，CF传递主要在记忆获取中发挥特殊而关键的作用，而不是在后续过程中。
