Nahar Mosammat Rezaun, Nasreen Syeda Anjuman, Paul Shyamal Kumar, Hoque Mohammed Abedul, Aung Meiji Soe, Haque Nazia, Hossain Tasmia, Tasnim Sheikh Anika, Tuly Sultana Jahan, Ishi Nashid Sultana, Ifa Iffat Ara, Mamun Abdullah Al, Arafa Parvez, Kobayashi Nobumichi
Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh.
International Medical College, Gazipur, Bangladesh.
IJID Reg. 2025 Jul 27;16:100714. doi: 10.1016/j.ijregi.2025.100714. eCollection 2025 Sep.
Nontuberculous mycobacteria (NTM) are an increasing cause of extrapulmonary infections affecting skin and soft tissue. This study aimed to determine the involvement of NTM in persistent surgical site infections (SSIs) in Bangladesh.
Specimens of SSIs (wound swab, pus, sinus discharge) were collected from patients who attended a tertiary care hospital during a 6-month period in 2024. NTM were detected by phenotypic methods (microscopic examination, culture, biochemical tests), immunochromatography, and polymerase chain reaction. Species of NTM were identified by sequence analysis of gene. Antimicrobial susceptibility was determined by broth microdilution test.
Among a total of 155 samples collected, NTM were detected in 12 samples (7.7%), which were identified to be (n = 5), (n = 4), , and (one specimen each). NTM were detected in patients aged 0-50 years who were showing variable durations of non-healing wound (3 to >14 weeks), more commonly after surgery of laparoscopic cholecystectomy and laparotomy. Resistance to amikacin, ciprofloxacin, and clarithromycin was noted for and .
NTM represented by and were revealed to be a significant cause of persistent SSIs of various patient groups in Bangladesh, associated with specific types of surgery.
非结核分枝杆菌（NTM）是影响皮肤和软组织的肺外感染日益增加的病因。本研究旨在确定NTM在孟加拉国持续性手术部位感染（SSI）中的作用。
2024年6个月期间，从一家三级护理医院就诊的患者中收集SSI标本（伤口拭子、脓液、窦道分泌物）。通过表型方法（显微镜检查、培养、生化试验）、免疫色谱法和聚合酶链反应检测NTM。通过基因序列分析鉴定NTM的种类。通过肉汤微量稀释试验确定抗菌药物敏感性。
在总共收集的155份样本中，12份样本（7.7%）检测到NTM，鉴定为（n = 5）、（n = 4）、、（各1份标本）。在0至50岁、伤口愈合时间不等（3至>14周）的患者中检测到NTM，更常见于腹腔镜胆囊切除术和剖腹手术后。和对阿米卡星、环丙沙星和克拉霉素耐药。
在孟加拉国，以和为代表的NTM是不同患者群体持续性SSI的重要病因，与特定类型的手术有关。
