Ratcliffe N A, Gagen S J
Tissue Cell. 1977;9(1):73-85. doi: 10.1016/0040-8166(77)90050-7.
Cellular aggregates or nodules which formed in Galleria mellonella larvae in response to injections of killed Bacillus cereus were examined during their first 24 hr of development. The initial aggregation stage was very rapid, occurring within 1 min, and was brought about by the release of an adhesive flocculent material by granular haemocytes in contact with the bacteria. This material, which subsequently became increasingly electron dense owing to melanin deposition, consolidated and by 5 min ramified all the intercellular spaces. The granular cells degenerated, releasing portions of cytoplasm form their peripheries, and eventurally appeared in the matrix as voids containing loosely flocculent material. No bacterial breakdown was seen, however. From 2-4 hr, plasmatocytes began to encapsulate the necrotic mass, and by 24 hr a complete sheath, composed of three discrete regions of cells, was formed. These findings are discussed in relation to the various proposed mechanisms of nodule and capsule formation.
对因注射灭活蜡样芽孢杆菌而在大蜡螟幼虫体内形成的细胞聚集体或小结在其发育的最初24小时内进行了检查。最初的聚集阶段非常迅速，在1分钟内发生，是由与细菌接触的颗粒血细胞释放出一种粘性絮凝物质引起的。这种物质随后由于黑色素沉积而电子密度越来越高，凝聚并在5分钟内分支遍布所有细胞间隙。颗粒细胞退化，从其周边释放出部分细胞质，最终在基质中呈现为含有松散絮凝物质的空隙。然而，未观察到细菌分解。从2至4小时起，浆细胞开始包裹坏死团块，到24小时时形成了一个由三个离散细胞区域组成的完整鞘。结合各种提出的小结和包囊形成机制对这些发现进行了讨论。
