群体感应淬灭：海洋放线菌提取物用于寻常痤疮控制的关键生物学活性。

Quorum quenching: A key biological activity of marine actinobacteria extracts for acne vulgaris control.

作者信息

De La Hoz-Romo María Clara, Díaz Luis, Gómez-León Javier, Reyes Luis Felipe, Quintero Marynes, Villamil Luisa

机构信息

Doctoral Program of Biosciences, School of Engineering, Universidad de La Sabana, Chía 250001, Colombia.

Bioprospecting Research Group, School of Engineering, Universidad de La Sabana, Chía 250001, Colombia.

出版信息

Biotechnol Rep (Amst). 2025 Jun 24;47:e00903. doi: 10.1016/j.btre.2025.e00903. eCollection 2025 Sep.

DOI:10.1016/j.btre.2025.e00903

PMID:40895590

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12391250/

Abstract

Quorum sensing (QS) regulates virulence factors in bacteria associated with acne vulgaris. Consequently, extracts from marine actinobacteria sourced from Colombian Caribbean sponges and octocorals were screened for quorum quenching (QQ) and anti-biofilm activity. The most promising extract, G12.218 from sp. CLIVUS-G12218, inhibited QS by 97 % via AI-2 signaling in BB170 and reduced biofilm by 60 % at 3 mg/L, without cytotoxicity to keratinocytes and fibroblasts. Untargeted metabolomics of QQ active and non-active extracts revealed distinct profiles enriched in terpenoids, phenols, and butenolides, potentially linked to bioactivity. These extracts also exhibited antioxidant capacity (18 ± 0.23 µmol TE/L) and hyaluronidase inhibition up to 72.8 %, suggesting skin-protective properties. These findings highlight the potential of marine actinobacteria as a source of QQ and anti-biofilm agents for acne therapy and skincare.

摘要

群体感应（QS）调节与寻常痤疮相关的细菌中的毒力因子。因此，对源自哥伦比亚加勒比海绵和八放珊瑚的海洋放线菌提取物进行了群体猝灭（QQ）和抗生物膜活性筛选。最有前景的提取物，来自CLIVUS-G12218菌株的G12.218，通过BB170中的AI-2信号传导抑制97%的群体感应，并在3 mg/L时使生物膜减少60%，对角质形成细胞和成纤维细胞无细胞毒性。对具有QQ活性和无活性提取物的非靶向代谢组学分析揭示了富含萜类化合物、酚类和丁烯内酯的不同谱图，可能与生物活性有关。这些提取物还表现出抗氧化能力（18±0.23 μmol TE/L）和高达72.8%的透明质酸酶抑制率，表明具有皮肤保护特性。这些发现突出了海洋放线菌作为用于痤疮治疗和皮肤护理的QQ和抗生物膜剂来源的潜力。