Mahgoub Samar M, Alawam Abdullah S, Rudayni Hassan A, Allam Ahmed A, Abdel Aziz Sahar Abdel Aleem, Eweis Abdullah A, Khaled Esraa, Shafei Rami, Mohamed Fatma, Mahmoud Rehab
Material Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University Beni-Suef 62511 Egypt
Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU) Riyadh 11623 Saudi Arabia
RSC Adv. 2025 Aug 29;15(37):30872-30899. doi: 10.1039/d5ra04212g. eCollection 2025 Aug 22.
This study developed a lysozyme-dequalinium chloride-loaded Zn-Fe layered double hydroxide (LDH)-chia seed mucilage matrix for enhanced antimicrobial efficacy and sustained drug delivery. The optimized formulation (15% w/v Zn-Fe LDH-chia seed hybrid) achieved encapsulation efficiencies of 93.30 ± 1.92% (lysozyme) and 88.74 ± 1.71% (dequalinium), with a particle size of 153.77 ± 5.79 nm, PDI of 0.18, and zeta potential of +26.44 ± 1.21 mV, ensuring stability and mucoadhesion. Surface morphology, structure, drug loading, and thermostability were confirmed using various techniques. release studies showed sustained drug release over 24 h. Antimicrobial assays demonstrated inhibition zones against (24.33 ± 2.03 mm), (14.67 ± 0.88 mm), (11.45 ± 0.887 mm), and (20.33 ± 2.03 mm). MIC/MBC analysis indicated high susceptibility of (MIC = 13.00 ± 2.60 μg mL, MBC = 20.83 ± 5.23 μg mL, < 0.001) and (MIC = 15.60 ± 1.05 μg mL, MBC = 26.07 ± 5.23 μg mL, < 0.01), with showing moderate sensitivity (MIC = 49.66 ± 4.32 μg mL, MBC = 72.93 ± 6.43 μg mL). MBC/MIC ratios below 4 confirmed bactericidal action. The formulation reduced biofilm formation by 90.1 ± 1.2% at the highest tested concentration. Cytotoxicity evaluation showed a CC50 of 306.6 μg mL, demonstrating improved cytocompatibility compared to the free components.
本研究开发了一种负载溶菌酶-氯化地喹氯铵的锌铁层状双氢氧化物（LDH）-奇亚籽黏液基质，以提高抗菌效果并实现药物的持续递送。优化后的配方（15% w/v锌铁LDH-奇亚籽混合物）对溶菌酶的包封率为93.30±1.92%，对氯化地喹氯铵的包封率为88.74±1.71%，粒径为153.77±5.79纳米，多分散指数为0.18，zeta电位为+26.44±1.21毫伏，确保了稳定性和黏膜黏附性。使用各种技术对表面形态、结构、载药量和热稳定性进行了确认。释放研究表明药物在24小时内持续释放。抗菌试验显示对金黄色葡萄球菌（抑菌圈为24.33±2.03毫米）、大肠杆菌（抑菌圈为14.67±0.88毫米）、铜绿假单胞菌（抑菌圈为11.45±0.887毫米）和白色念珠菌（抑菌圈为20.33±2.03毫米）有抑菌圈。最低抑菌浓度/最低杀菌浓度分析表明金黄色葡萄球菌（最低抑菌浓度=13.00±2.60微克/毫升，最低杀菌浓度=20.83±5.23微克/毫升，P<0.001）和大肠杆菌（最低抑菌浓度=15.60±1.05微克/毫升，最低杀菌浓度=26.07±5.23微克/毫升，P<0.01）高度敏感，铜绿假单胞菌表现出中等敏感性（最低抑菌浓度=49.66±4.32微克/毫升，最低杀菌浓度=72.93±6.43微克/毫升）。最低杀菌浓度/最低抑菌浓度比值低于4证实了杀菌作用。在最高测试浓度下，该配方使白色念珠菌生物膜形成减少了90.1±1.2%。细胞毒性评估显示半数细胞毒性浓度为306.6微克/毫升，表明与游离成分相比，细胞相容性有所改善。
