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放线菌介导的使用sp. ISP-2 27菌株绿色合成CuO/Zn-Al层状双氢氧化物纳米复合材料：增强抗菌活性的协同研究

Actinobacterium-Mediated Green Synthesis of CuO/Zn-Al LDH Nanocomposite Using sp. ISP-2 27: A Synergistic Study that Enhances Antimicrobial Activity.

作者信息

Eweis Abdullah A, Ahmad Maged S, El Domany Ehab B, Al-Zharani Mohammed, Mubarak Mohammed, E Eldin Zienab, GadelHak Yasser, Mahmoud Rehab, Hozzein Wael N

机构信息

Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt.

Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef 62511, Egypt.

出版信息

ACS Omega. 2024 Aug 2;9(32):34507-34529. doi: 10.1021/acsomega.4c02133. eCollection 2024 Aug 13.

DOI:10.1021/acsomega.4c02133
PMID:39157139
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11325407/
Abstract

Bacterial resistance to conventional antibiotics has created an urgent need to develop enhanced alternatives. Nanocomposites combined with promising antibacterial nanomaterials can show improved antimicrobial activity compared to that of their components. In this work, green synthesized CuO nanoparticles (NPs) supported on an anionic clay with a hydrotalcite-like structure such as Zn-Al layered double hydroxide (LDH) nanocomposite were investigated as antimicrobial agents. This nanocomposite was synthesized using sp. ISP-2 27 cell-free supernatant to form CuO NPs on the surface of previously synthesized LDH. The prepared samples were characterized using UV-Vis spectrophotometry, XRD, FTIR, Field emission scanning electron microscopy with EDX, zeta potential, and hydrodynamic particle size. UV-vis spectral analysis of the biosynthesized CuO NPs revealed a maximum peak at 300 nm, indicating their successful synthesis. The synthesized CuO NPs had a flower-like morphology with a size range of 43-78 nm, while the LDH support had a typical hexagonal layered structure. The zeta potentials of the CuO NPs, Zn-Al LDH, and CuO NPs/LDH nanocomposite were -21.4, 22.3, and 30.8 mV, respectively, while the average hydrodynamic sizes were 687, 735, and 528 nm, respectively. The antimicrobial activity of the produced samples was tested against several microbes. The results demonstrated that the nanocomposite displayed superior antimicrobial properties compared to those of its components. Among the microbes tested, ATCC 7644 was more sensitive (30 ± 0.34) to the biosynthesized nanocomposite than to CuO NPs (25 ± 0.05) and Zn-Al LDH (22 ± 0.011). In summary, the use of nanocomposites with superior antimicrobial properties has the potential to offer innovative solutions to the global challenge of antibiotic resistance by providing alternative treatments, reducing the reliance on traditional antibiotics, and contributing to the development of more effective and targeted therapeutic approaches.

摘要

细菌对传统抗生素的耐药性使得迫切需要开发更有效的替代物。与有前景的抗菌纳米材料结合的纳米复合材料相比其组分可表现出更好的抗菌活性。在这项工作中，研究了负载在具有类水滑石结构的阴离子粘土如锌铝层状双氢氧化物（LDH）纳米复合材料上的绿色合成氧化铜纳米颗粒（NPs）作为抗菌剂。该纳米复合材料是利用嗜麦芽窄食单胞菌ISP-2 27的无细胞上清液在先前合成的LDH表面形成氧化铜纳米颗粒而合成的。使用紫外可见分光光度法、X射线衍射、傅里叶变换红外光谱、带能谱的场发射扫描电子显微镜、zeta电位和流体动力学粒径对制备的样品进行了表征。对生物合成的氧化铜纳米颗粒的紫外可见光谱分析显示在300nm处有一个最大峰，表明其成功合成。合成的氧化铜纳米颗粒具有花状形态，尺寸范围为43-78nm，而LDH载体具有典型的六方层状结构。氧化铜纳米颗粒、锌铝LDH和氧化铜纳米颗粒/LDH纳米复合材料的zeta电位分别为-21.4、22.3和30.8mV，而平均流体动力学尺寸分别为687、735和528nm。对制备的样品针对几种微生物测试了抗菌活性。结果表明，该纳米复合材料与其组分相比显示出优异的抗菌性能。在所测试的微生物中，美国典型培养物保藏中心7644对生物合成的纳米复合材料（30±0.34）比对氧化铜纳米颗粒（25±0.05）和锌铝LDH（22±0.011）更敏感。总之，使用具有优异抗菌性能的纳米复合材料有潜力通过提供替代治疗、减少对传统抗生素的依赖以及促进更有效和有针对性的治疗方法的开发，为全球抗生素耐药性挑战提供创新解决方案。

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