Sutthi Nantaporn, Karirat Thipphiya, Promjamorn Piyathida, Ma Nyuk Ling, Luang-In Vijitra
Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Applied Animal and Aquatic Sciences Research Unit, Division of Fisheries, Faculty of Technology, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Data Brief. 2025 Aug 19;62:111994. doi: 10.1016/j.dib.2025.111994. eCollection 2025 Oct.
This study aimed to evaluate the optimal nitrogen source for the production of antioxidant exopolysaccharides (EPSs) by KW8 using cassava pulp carbon source. Various nitrogen sources were initially screened, and yeast extract was identified as the most effective. Further optimization was conducted using Response Surface Methodology (RSM) with a Central Composite Design (CCD) to assess the influence of key parameters, including pH, inoculum size, carbon-to-nitrogen (C/N) ratio, and agitation speed on EPS production. The amount of EPS, media pH, reducing sugar level, and viable bacterial population after 3 days of fermentation were recorded. The EPS's structural characteristics were investigated using scanning electron microscopy (SEM). The levels of bioactive compounds and antioxidant capabilities in the EPS, such as its total phenolic content, total flavonoid content, ferric reducing antioxidant power, hydroxyl radical scavenging activity, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were also measured. This experiment used microbial cell factories to convert cassava pulp, an agro-biowaste, into more valuable EPS bioproducts, which supports sustainable developmental goals.
本研究旨在评估KW8利用木薯浆碳源生产抗氧化胞外多糖（EPSs）的最佳氮源。最初筛选了各种氮源，酵母提取物被确定为最有效的氮源。使用响应面法（RSM）和中心复合设计（CCD）进行进一步优化，以评估关键参数（包括pH值、接种量、碳氮比（C/N）和搅拌速度）对EPS生产的影响。记录发酵3天后EPS的产量、培养基pH值、还原糖水平和活菌数。使用扫描电子显微镜（SEM）研究EPS的结构特征。还测定了EPS中生物活性化合物的含量和抗氧化能力，如总酚含量、总黄酮含量、铁还原抗氧化能力、羟自由基清除活性和2,2-二苯基-1-苦基肼（DPPH）清除活性。本实验利用微生物细胞工厂将农业生物废弃物木薯浆转化为更有价值的EPS生物产品，这有助于实现可持续发展目标。
