Karirat Thipphiya, Deeseenthum Sirirat, Ma Nyuk Ling, Sutthi Nantaporn, Luang-In Vijitra
Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham, Thailand.
Biological Security and Sustainability (BioSES) Research Interest Group, Terengganu, Malaysia.
Nat Prod Res. 2024 Sep 2:1-4. doi: 10.1080/14786419.2024.2398720.
This study tested the antioxidant activities of exopolysaccharides (EPSs) produced by eight spp. from Thai milk kefir utilising four agricultural by-products in Thailand; copra meal, mangosteen peel, sorghum, and para rubber sawdust as carbon sources. Sorghum showed the highest starch and sugar content of 73.33% while copra meal showed the lowest (13.08%). PS21 produced the highest dry weight EPS, followed by KW1 and PS22 from four substrates. PS21 generated the most EPS with sorghum (0.75 ± 0.09 g DW/100 mL culture), followed by mangosteen peel (0.61 ± 0.07 g). EPS from KW1 using copra meal displayed the highest DPPH radical scavenging activity of 33.39 ± 1.34% and EPS from the same bacteria using sorghum displayed the highest hydroxyl radical scavenging activity of 49.78 ± 0.86%. This approach demonstrated a bio-circular green economy paradigm in converting agricultural biowastes into valuable EPS biomaterials with potential applications.
本研究测试了利用泰国四种农业副产品（椰粕、山竹果皮、高粱和橡胶木屑）作为碳源，由泰国开菲尔粒中的8个菌株产生的胞外多糖（EPSs）的抗氧化活性。高粱的淀粉和糖含量最高，为73.33%，而椰粕的含量最低（13.08%）。PS21产生的EPS干重最高，其次是来自四种底物的KW1和PS22。PS21利用高粱产生的EPS最多（0.75±0.09 g DW/100 mL培养物），其次是山竹果皮（0.61±0.07 g）。使用椰粕的KW1产生的EPS表现出最高的DPPH自由基清除活性，为33.39±1.34%，而使用高粱的同一细菌产生的EPS表现出最高的羟基自由基清除活性，为49.78±0.86%。这种方法展示了一种生物循环绿色经济模式，即将农业生物废弃物转化为具有潜在应用价值的EPS生物材料。
