Lacouture Aurélie, Sylla Mame Sokhna, Germain Lucas, Audet-Walsh Étienne
Department of Molecular Medicine, Faculty of Medicine, Université Laval, Québec City, Canada.
Endocrinology - Nephrology Research Division, Centre de recherche du CHU de Québec - Université Laval, Québec City, Canada.
Data Brief. 2025 Aug 14;62:111984. doi: 10.1016/j.dib.2025.111984. eCollection 2025 Oct.
The mammary gland development and during life is strongly regulated by hormones. To study the genes regulated specifically by the estrogen signalling pathway in the epithelial compartment, we treated mouse mammary epithelial organoids with estradiol, the most potent endogenous estrogen. At maturity, after 11 days of treatment, organoids were collected, and RNA was purified for next-generation sequencing. The bulk mRNA-seq data obtained were verified for raw quality, and reads were pseudo-aligned on the murine reference transcriptome (Gencode vM25). Differentially expressed genes were identified using DESeq2 to gain a better understanding of the impact of estrogens on the mammary epithelial cell transcriptome . These data can be reanalyzed and combined with recent single-cell RNA-seq data to study the estrogen-dependent transcriptome at the cellular level and better understand the functional impact on the mammary gland in physiopathological conditions, such as during lactation, following endocrine-disrupting chemical exposure, or through the course of carcinogenesis.
乳腺在整个生命过程中的发育受到激素的强烈调控。为了研究雌激素信号通路在上皮细胞区室中特异性调控的基因，我们用雌二醇（最有效的内源性雌激素）处理小鼠乳腺上皮类器官。在处理11天后，类器官成熟，收集类器官，并纯化RNA用于下一代测序。对获得的大量mRNA-seq数据进行原始质量验证，并将读数伪比对到小鼠参考转录组（Gencode vM25）上。使用DESeq2鉴定差异表达基因，以更好地了解雌激素对乳腺上皮细胞转录组的影响。这些数据可以重新分析，并与最近的单细胞RNA-seq数据相结合，以在细胞水平上研究雌激素依赖性转录组，并更好地了解在生理病理条件下，如哺乳期、接触内分泌干扰化学物质后或在致癌过程中，对乳腺的功能影响。
