Mendoza-Dasilva Maria V, Rodriguez-Rivera Lorraine, Rodriguez-Fernandez Imilce A
Biology, University of Puerto Rico at Río Piedras, San Juan, San Juan, Puerto Rico.
Natural Sciences, InterAmerican University of Puerto Rico, San Juan Campus, Puerto Rico.
MicroPubl Biol. 2025 Aug 16;2025. doi: 10.17912/micropub.biology.001615. eCollection 2025.
Lactic acid bacteria (LAB) are widely used as probiotics and in fermented foods, yet their antibiotic resistance profiles remain under-characterized. This study evaluates the antibiotic resistance profiles of two ( ) strains, LpWF (isolated from ) and Lp39 (from cabbage), using broth microdilution methods. Minimum inhibitory concentrations (MICs) and minimum lethal concentrations (MLCs) were determined for six antibiotics. Strain-specific differences were found in MICs for kanamycin, neomycin, and geneticin, while ampicillin and chloramphenicol MICs were similar. Both strains exhibited intrinsic resistance to vancomycin. These findings contribute to efforts to characterize the LAB resistome and inform probiotic strain selection.
乳酸菌（LAB）被广泛用作益生菌和用于发酵食品，但它们的抗生素抗性谱仍未得到充分表征。本研究使用肉汤微量稀释法评估了两株乳酸菌菌株LpWF（从[具体来源未给出]分离）和Lp39（从卷心菜中分离）的抗生素抗性谱。测定了六种抗生素的最低抑菌浓度（MIC）和最低致死浓度（MLC）。在卡那霉素、新霉素和庆大霉素的MIC方面发现了菌株特异性差异，而氨苄青霉素和氯霉素的MIC相似。两株菌株均表现出对万古霉素的固有抗性。这些发现有助于对乳酸菌抗性组进行表征，并为益生菌菌株的选择提供参考。
