Mansour Yasmine A, Youssef Fady Sayed, Hussein Shaymaa, AbdEl Halim Hanan F, Ismail Sameh H, Mohamed Gehad G, Abdelfattah Abdelfattah M, Ezz-Eldin Rasha M M, Mahboub Heba H, Ibrahim Sherin
Cytology and Histology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12411, Egypt.
Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Tissue Cell. 2025 Aug 20;98:103095. doi: 10.1016/j.tice.2025.103095.
The present novel trial assesses the prophylactic influence of ZnO NPs in comparison to silymarin against liver damage induced by acetaminophen (APAP). Forty albino rats were allocated into 4 groups (n = `10 rats/ group). Group I (Control), was orally administered 0.9 % NaCl for 21 days. Group II (exposed to APAP) received distilled water (1 ml/kg per day) via oral gavage for 19 successive days followed by APAP (600 mg/kg, PO) twice daily for 2 days. Group III (APAP+ Silymarin) was orally administered silymarin at the dose of 200 mg/kg daily for 19 days followed by APAP (600 mg/kg) via oral gavage twice daily for 2 successive days. Group IV (APAP+ ZnO NPs) was orally given ZnO NPs at the dose of 100 mg/kg daily for 19 days followed by (600 mg/kg/ twice daily) APAP orally for 2 successive days. APAP exhibited a substantial elevation of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, nitric oxide and malondialdehyde levels. Meanwhile, APAP markedly decreased catalase, SOD, GST and TAC levels. A significant elevation in DNA damage inside hepatocytes was noticed. APAP induced many histopathological changes. Conversely, the prophylactic use of ZnO NPs inhibited the elevation of AST, ALT, ALP, NO and MDA as well as the decline of catalase, SOD, GST and TAC levels induced by APAP toxicity compared to group III. Marked improvement of the architecture of hepatic tissue was noticed in group IV. In conclusion, ZnO-NPs were more actual in ameliorating APAP hepatotoxicity compared to silymarin via antioxidant and antiapoptotic pathways.
本新型试验评估了氧化锌纳米颗粒（ZnO NPs）与水飞蓟宾相比，对乙酰氨基酚（APAP）诱导的肝损伤的预防作用。40只白化大鼠被分为4组（每组n = 10只大鼠）。第一组（对照组）口服0.9%氯化钠溶液，持续21天。第二组（暴露于APAP）连续19天通过口服灌胃给予蒸馏水（1毫升/千克/天），随后连续2天每天两次口服APAP（600毫克/千克）。第三组（APAP + 水飞蓟宾）在19天内每天口服200毫克/千克剂量的水飞蓟宾，随后连续2天每天两次通过口服灌胃给予APAP（600毫克/千克）。第四组（APAP + ZnO NPs）在19天内每天口服100毫克/千克剂量的ZnO NPs，随后连续2天每天两次口服（600毫克/千克）APAP。APAP使丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶、一氧化氮和丙二醛水平大幅升高。同时，APAP显著降低了过氧化氢酶、超氧化物歧化酶、谷胱甘肽S-转移酶和总抗氧化能力水平。肝细胞内DNA损伤显著增加。APAP诱导了许多组织病理学变化。相反，与第三组相比，预防性使用ZnO NPs抑制了APAP毒性诱导的AST、ALT、ALP、NO和MDA水平的升高以及过氧化氢酶、超氧化物歧化酶、谷胱甘肽S-转移酶和总抗氧化能力水平的下降。第四组肝组织结构明显改善。总之，与水飞蓟宾相比，ZnO NPs通过抗氧化和抗凋亡途径在改善APAP肝毒性方面更有效。
