Giang Nguyen Ngan, Linh Le Thi Thuy, Tien Trinh Thi Thuy, Nga Pham Thi, Zhang Xin Rui, Jin Yong Xun, Zhou Shu Yi, Kwon Han Jin, Ham Jung Ryul, Lee Won Ku, Gu Yeon Ju, Lee Yong Hyun, Chien Pham Ngoc, Heo Chan Yeong
Department of Medical Device Development, College of Medicine, Seoul National University, Seoul, Republic of Korea.
Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam, Korea.
Aesthetic Plast Surg. 2025 Sep 2. doi: 10.1007/s00266-025-05195-y.
Dermal fillers have become prevalent in aesthetic surgery, offering noninvasive solutions for addressing signs of aging and enhancing facial features.
In this study, the in vivo administration of dermal fillers, including a hyaluronic-cross-linked filler (HA-R), a PDLLA-HA combined filler (HA-PDLLA), a microparticle PDO filler (PDO), a PN filler extracted from salmon milt (PN), and pure hyaluronic acid (HA), along with PBS as a control, was evaluated over a 12 week period.
HA-R exhibited sustained volume retention, contrasting with rapid volume loss observed with PN, and gradual dissolution of PDO by week 12. Histological evaluation revealed varying inflammatory responses, with PDO showing notable alleviation of inflammation after 8 weeks. MT staining demonstrated increased collagen formation post-injection, peaking at 4 weeks for most fillers. Immunofluorescent staining highlighted the initial promotion of collagen synthesis by PDO and HA-PDLLA, followed by a subsequent decline after 8 weeks.
This study provided comprehensive insights into the biophysical properties and efficacy of various dermal fillers in stimulating collagen production. PDO and HA-PDLLA initially demonstrated efficacy in promoting collagen synthesis, but limitations were noted in sustaining long-term collagen production. Understanding the dynamics of filler administration and its impact on tissue response is essential for optimizing outcomes in aesthetic surgery practice.
This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies.
真皮填充剂在美容手术中已变得很普遍，为解决衰老迹象和改善面部特征提供了非侵入性解决方案。
在本研究中，对包括透明质酸交联填充剂（HA-R）、聚-D,L-乳酸-透明质酸复合填充剂（HA-PDLLA）、微粒聚对二氧环己酮填充剂（PDO）、从鲑鱼精液中提取的PN填充剂（PN）和纯透明质酸（HA）在内的真皮填充剂进行体内给药，并以PBS作为对照，评估为期12周。
HA-R表现出持续的体积保留，与PN观察到的快速体积损失形成对比，且到第12周时PDO逐渐溶解。组织学评估显示出不同的炎症反应，PDO在8周后炎症明显减轻。MT染色显示注射后胶原形成增加，大多数填充剂在4周时达到峰值。免疫荧光染色突出显示PDO和HA-PDLLA最初促进胶原合成，随后在8周后下降。
本研究全面深入了解了各种真皮填充剂在刺激胶原产生方面的生物物理特性和功效。PDO和HA-PDLLA最初在促进胶原合成方面显示出功效，但在维持长期胶原产生方面存在局限性。了解填充剂给药动态及其对组织反应的影响对于优化美容手术实践结果至关重要。
本期刊要求作者为每篇适用循证医学排名的投稿指定证据级别。这排除了综述文章、书评以及涉及基础科学、动物研究、尸体研究和实验研究的手稿。
