Kobayashi J, Nakamura H, Ohizumi Y
Toxicon. 1985;23(5):783-9. doi: 10.1016/0041-0101(85)90009-1.
An active component (MAC) of Conus magus venom has been purified by gel filtration and ion exchange chromatographies, monitored by cardiotonic activity in the guinea-pig isolated left atria. MAC was estimated to be a protein of mol. wt between 45,000 and 65,000 by gel filtration and protease experiments. The purified protein at concentrations above 10(-7) g/ml elicited powerful rhythmic contractions of the guinea-pig vas deferens, rhythmic transient contractions of the ileum followed by relaxations, and a marked increase in the contractile force of the left atria. These responses to the toxin were abolished by tetrodotoxin. These results suggest that the action of MAC on the ileum and vas deferens may be mediated via nerves. It is also suggested that the major mechanism of action is due to activation of Na channels on the cell membrane.
通过凝胶过滤和离子交换色谱法纯化了芋螺毒液的一种活性成分（MAC），以豚鼠离体左心房的强心活性进行监测。通过凝胶过滤和蛋白酶实验估计MAC是一种分子量在45,000至65,000之间的蛋白质。浓度高于10^(-7) g/ml的纯化蛋白可引起豚鼠输精管强烈的节律性收缩、回肠的节律性短暂收缩并随后松弛，以及左心房收缩力显著增加。河豚毒素可消除对该毒素的这些反应。这些结果表明，MAC对回肠和输精管的作用可能是通过神经介导的。还表明其主要作用机制是由于细胞膜上钠通道的激活。
