Kim Naeun, Kim Yoonjeong, Yu Jihyeon, Park Eunji, Choi Youngmin, Kim Younghwa
Department of Food Science and Biotechnology, BB21plus Project Team, Kyungsung University, Busan 48434, Korea.
Food Contaminants Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Chungbuk 28159, Korea.
Prev Nutr Food Sci. 2025 Aug 31;30(4):399-408. doi: 10.3746/pnf.2025.30.4.399.
This study aimed to evaluate the extraction of biotin from plant-based foods using takadiastase in combination with autoclave- or ultrasound-assisted extraction. In cereals, vegetables, and mushrooms, autoclave-assisted enzymatic extraction obtained higher analytical values compared with autoclave-assisted extraction. However, in legumes and nuts, ultrasound-assisted enzymatic extraction obtained higher biotin content compared with either autoclave- or ultrasound-assisted extraction. Furthermore, method validation for the biotin analysis was performed to ensure the reliability of biotin extraction procedures from plant-based foods. The results demonstrated excellent linearity (R=0.9999), accuracy (95.024%－102.507% recovery), and precision (1.158%－4.724% relative standard deviation), with limits of detection and quantification of 0.009 and 0.025 µg/100 g, respectively. These findings could provide reliable data on the biotin content of commonly consumed plant-based foods in Korea.
本研究旨在评估使用淀粉酶结合高压釜辅助或超声辅助提取法从植物性食物中提取生物素的效果。在谷物、蔬菜和蘑菇中，与高压釜辅助提取相比，高压釜辅助酶解提取获得了更高的分析值。然而，在豆类和坚果中，与高压釜辅助提取或超声辅助提取相比，超声辅助酶解提取获得了更高的生物素含量。此外，还对生物素分析进行了方法验证，以确保从植物性食物中提取生物素程序的可靠性。结果显示出良好的线性（R=0.9999）、准确度（回收率为95.024%－102.507%）和精密度（相对标准偏差为1.158%－4.724%），检测限和定量限分别为0.009和0.025 µg/100 g。这些发现可为韩国常见消费植物性食物的生物素含量提供可靠数据。
