Liu Ying, Lei Zhen, Ren Congcong, Mao Jifu, Zhao Fan, Wang Lu, Guan Guoping
Shanghai Frontier Science Research Center for Advanced Textiles, College of Textiles, Donghua University, Shanghai, 201620, China; Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai, 201620, China.
Shanghai Frontier Science Research Center for Advanced Textiles, College of Textiles, Donghua University, Shanghai, 201620, China; Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai, 201620, China.
Anal Chim Acta. 2025 Oct 22;1372:344454. doi: 10.1016/j.aca.2025.344454. Epub 2025 Jul 21.
Blood glucose monitoring is of paramount importance for patients with diabetes. However, conventional glucose meters exhibit limited accuracy and present challenges for prolonged use, primarily due to the high consumption of glucose oxidase (GOD) and their sensitivity to temperature and environmental factors. phenylboronic acid (PBA), benefiting from its reversible reaction with glucose and superior stability, holds potential as a promising medium for long-term glucose monitoring.
In this study, a microneedle substrate with low solubilization loss was obtained for the detection of glucose levels in interstitial fluids (ISF) by co-mixing silk fibroin and proline (Pro) as a PBA carrier. This microneedle sensor demonstrated a sensitive and linear response to glucose concentrations ranging from 1 to 20 mM in vitro, exhibiting correlation coefficients of 0.999 for the range of 1-12 mM and 0.997 for 12-20 mM. It displayed enhanced stability and a prolonged monitoring duration when compared to GOD-based commercial glucose meters. Additionally, it was unaffected by environmental factors such as temperature and pH, and demonstrated strong interference resistance. It successfully achieved real-time, long-term glucose concentration monitoring for up to 21 days (only ∼14 days for GOD-based measurement).
This study presents a novel approach to the development of wearable glucose monitoring devices characterized by extended duration and enhanced stability. We believe that this work makes an exciting and important contribution to the development of stable, highly sensitive and long-term glucose monitoring devices with the potential to significantly improve diabetes management.
血糖监测对糖尿病患者至关重要。然而，传统血糖仪的准确性有限，且长期使用存在挑战，主要原因是葡萄糖氧化酶（GOD）消耗量大，以及对温度和环境因素敏感。苯硼酸（PBA）因其与葡萄糖的可逆反应和卓越的稳定性，有望成为长期血糖监测的理想介质。
在本研究中，通过将丝素蛋白和脯氨酸（Pro）作为PBA载体共混，获得了一种溶解损失低的微针基底，用于检测间质液（ISF）中的葡萄糖水平。该微针传感器在体外对1至20 mM的葡萄糖浓度表现出灵敏的线性响应，在1 - 12 mM范围内的相关系数为0.999，在12 - 20 mM范围内为0.997。与基于GOD的商用血糖仪相比，它具有更高的稳定性和更长的监测持续时间。此外，它不受温度和pH等环境因素的影响，具有很强的抗干扰能力。它成功实现了长达21天的实时、长期葡萄糖浓度监测（基于GOD的测量仅约14天）。
本研究提出了一种开发可穿戴葡萄糖监测设备的新方法，其特点是持续时间延长和稳定性增强。我们相信，这项工作为开发稳定、高灵敏度和长期葡萄糖监测设备做出了令人兴奋且重要的贡献，有望显著改善糖尿病管理。
