Sun Hongyi, Wang Shidi, Zhu Anwei, Wang Tao, Wang Haoxin, Zheng Youbin, Shi Guoyue, Zhang Min
School of Chemistry and Molecular Engineering, In Situ Devices Research Center, East China Normal University, Shanghai 200241, China.
Shanghai Key Laboratory of Intelligent Sensing and Detection Technology, School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, China.
ACS Sens. 2025 Jul 25;10(7):4906-4916. doi: 10.1021/acssensors.5c00271. Epub 2025 Jul 15.
Personalized healthcare requires convenient and high-fidelity approaches to monitoring metabolic biomarkers, for example, uric acid (UA), toward next-generation disease management. Although wearable microneedle-based devices stand out with miniaturized needle-like sensors capable of transdermal monitoring of UA in interstitial fluid (ISF) in an imperceptible manner, their rigid nature and limited lifespan pose considerable challenge to the practicability as continuous biosensors in real-world application. Here, we present a wearable microneedle-based biosensor that integrates the strength of microneedle with a Prussian Blue analog, that is, nickel hexacyanoferrate, enhanced sensing interface to minimally invasive access UA in ISF and enable highly blood-correlated, continuous, and long-term monitoring. By incorporating conductive poly(2,6-diaminopyridine), the as-proposed microneedle biosensor renders highly sensitive monitoring of UA with a detection limit of 1.78 μM. In addition, the fully integrated device features prominent mechanical resilience and flexibility against undesirable physical deformations, ensuring reliable sensing performance of UA in daily activities. In animal models, the resulting biosensors achieved dynamic UA tracking with excellent stability and captured prolonged amperometric information interpreting the metabolic changes of UA levels during a seven-week experiment. The strong correlation between the obtained ISF and blood UA levels demonstrated the high analytical accuracy of the biosensor, which, thus, provided a powerful approach to fulfill personalized UA management in the future.
个性化医疗需要便捷且高保真的方法来监测代谢生物标志物，例如尿酸（UA），以实现下一代疾病管理。尽管基于可穿戴微针的设备凭借能够以不可察觉的方式对间质液（ISF）中的UA进行透皮监测的小型针状传感器脱颖而出，但其刚性本质和有限的使用寿命对作为连续生物传感器在实际应用中的实用性构成了相当大的挑战。在此，我们展示了一种基于可穿戴微针的生物传感器，它将微针的优势与普鲁士蓝类似物（即六氰合铁酸镍）相结合，增强了传感界面，以微创方式获取ISF中的UA，并实现高度与血液相关的连续长期监测。通过掺入导电聚（2,6 - 二氨基吡啶），所提出的微针生物传感器对UA的监测具有高灵敏度，检测限为1.78 μM。此外，完全集成的设备具有出色的机械弹性和柔韧性，可抵抗不良物理变形，确保在日常活动中对UA具有可靠的传感性能。在动物模型中，所得生物传感器实现了对UA的动态跟踪，具有出色的稳定性，并在为期七周的实验中获取了长时间的安培信息，解释了UA水平的代谢变化。所获得的ISF和血液UA水平之间的强相关性证明了生物传感器的高分析准确性，因此，它为未来实现个性化UA管理提供了一种有力的方法。
