Khaduev S Kh, Lukasheva E V, Smirnova I P, Berezov T T
Vopr Med Khim. 1985 Sep-Oct;31(5):130-4.
An improved and relatively rapid procedure is developed for isolation and purification of a new antitumor enzyme L-lysyl-alpha-oxidase from Trichoderma sp. The method involves four steps, instead of six steps described previously, with a yield of 22.4%. The purified enzyme preparation was homogeneous as shown by polyacrylamide gel disc electrophoresis and ultracentrifugation. Physico-chemical and antitumor properties of the enzyme are under study.
已开发出一种改进的、相对快速的程序，用于从木霉属真菌中分离和纯化一种新的抗肿瘤酶L-赖氨酰-α-氧化酶。该方法包括四个步骤，而非先前所述的六个步骤，产率为22.4%。如聚丙烯酰胺凝胶圆盘电泳和超速离心所示，纯化的酶制剂是均一的。该酶的物理化学性质和抗肿瘤特性正在研究中。
