Ivanova E M, Nikiforova A A, Alksnis E G
Vopr Med Khim. 1985 Nov-Dec;31(6):123-6.
A procedure is described for production of artificial substrate, containing except of cholesterol and lecithin as well as the protein moiety, used in estimation of the rate of lecithin-cholesterol-acyltransferase reaction in whole blood plasma. The protein component of proteoliposomes involved total fraction of apoproteins of high density lipoproteins (HDL). The proteins containing in proteoliposomes were obtained by means of chloroform-methanol delipidation of the total HDL fraction, isolated from human blood plasma using preparative ultracentrifugation. Optimal concentration of the apoprotein in proteoliposomes was determined.
描述了一种用于生产人工底物的方法，该底物除胆固醇、卵磷脂以及蛋白质部分外，还用于估计全血血浆中卵磷脂 - 胆固醇 - 酰基转移酶反应的速率。蛋白脂质体的蛋白质成分涉及高密度脂蛋白（HDL）载脂蛋白的总部分。蛋白脂质体中含有的蛋白质是通过对从人血浆中使用制备性超速离心分离出的总HDL部分进行氯仿 - 甲醇脱脂获得的。确定了蛋白脂质体中载脂蛋白的最佳浓度。
