Cheng Hanghang, Chen Wentao, Deng Zejun, Yin Minghui, Zhuang Chengyu, Qi Zhi-Mei, Xue Ning, Liu Chunxiu
State Key Laboratory of Transducer Technology, Aerospace Information Research Institute (AIR), Chinese Academy of Sciences Beijing China
University of Chinese Academy of Sciences Beijing China.
RSC Adv. 2025 Sep 2;15(37):30969-30979. doi: 10.1039/d5ra05254h. eCollection 2025 Aug 22.
The trace-level detection of proteins in the human body holds significant importance for health monitoring and disease prevention. To conduct real-time monitoring of the human body's health status, both wearable and implantable devices require label-free sensing. However, the signals of a considerable number of label-free electrochemical sensors originate from the signal capture medium. These signals are weak and are prone to being lost or masked by other signals. We report a Prussian blue (PB)-based self-redox aptamer sensor was developed, enabling real-time human serum albumin (HSA) detection without external redox mediators. The designed sensing interface successfully achieved the quantitative detection of HSA in PBS with a sensitivity of 124 fg mL. In addition, to validate universality, the sensor was tested with retinol-binding protein (RBP4), showing comparable performance. This label-free electrochemical sensor can achieve one-step detection without additional reagents. This system has application potential in on-site detection, as well as wearable sensing.
人体中蛋白质的痕量检测对健康监测和疾病预防具有重要意义。为了对人体健康状况进行实时监测，可穿戴和植入式设备都需要无标记传感。然而，相当数量的无标记电化学传感器的信号源自信号捕获介质。这些信号很微弱，容易被其他信号丢失或掩盖。我们报道了一种基于普鲁士蓝（PB）的自氧化还原适体传感器，该传感器能够在无需外部氧化还原介质的情况下实时检测人血清白蛋白（HSA）。所设计的传感界面成功实现了在PBS中对HSA的定量检测，灵敏度为124 fg/mL。此外，为了验证通用性，该传感器用视黄醇结合蛋白（RBP4）进行了测试，表现出可比的性能。这种无标记电化学传感器无需额外试剂即可实现一步检测。该系统在现场检测以及可穿戴传感方面具有应用潜力。
