Schmidt R, Meinel P
Acta Histochem. 1977;58(2):311-6.
Metal-containing secretory granules of alpha 2-cells of the LANGERHANS' islets of normal and alloxan-diabetic rats were stained for electron microscopy by a special method. Thereafter the electron micrographs were analysed by the Quantimet 720 image analysing computer with respect to size of the granules and their quantitative distribution in classes. The following statistical tests verified significant differences between the distributions of the secretory granules under normal and diabetic conditions. The secretory granules from alloxan-diabetic animals were diminished distinctly. The examination by image analysing computer confirmed objectively observations, we have made and published formerly.
采用一种特殊方法对正常大鼠和四氧嘧啶糖尿病大鼠胰岛α2细胞中含金属的分泌颗粒进行电子显微镜染色。之后，使用Quantimet 720图像分析计算机对电子显微照片的颗粒大小及其在类别中的定量分布进行分析。以下统计检验证实了正常和糖尿病条件下分泌颗粒分布之间的显著差异。四氧嘧啶糖尿病动物的分泌颗粒明显减少。通过图像分析计算机进行的检查客观地证实了我们之前已经做出并发表的观察结果。
