[Computer analyse of ultrahistochemical observed metal containing secretory granules in Langerhans islets cells of normoglycemic and diabetic rat].

Metal-containing secretory granules of alpha 2-cells of the LANGERHANS' islets of normal and alloxan-diabetic rats were stained for electron microscopy by a special method. Thereafter the electron micrographs were analysed by the Quantimet 720 image analysing computer with respect to size of the granules and their quantitative distribution in classes. The following statistical tests verified significant differences between the distributions of the secretory granules under normal and diabetic conditions. The secretory granules from alloxan-diabetic animals were diminished distinctly. The examination by image analysing computer confirmed objectively observations, we have made and published formerly.