Hunt Benjamin, Hunt Timothy, Xuan Zhao
School of Biology and Ecology, University of Maine, Orono, Maine, United States.
MicroPubl Biol. 2025 Aug 19;2025. doi: 10.17912/micropub.biology.001712. eCollection 2025.
The active zone gene encodes three main isoforms. The long isoform, CLA-1 L, functions beyond regulating synaptic vesicle exocytosis, including synaptic vesicle clustering and endocytic sorting of a transmembrane autophagy protein. CLA-1 L contains a large N-terminal repetitive region. Sequence analysis indicates that this region is enriched with acidic residues and displays disordered structures interspersed with helical sections. While modeling a portion of the repetitive region revealed dynamic transitions between compact and less compact conformations, modeling the entire region suggests a predominantly extended structure. This study sheds light on how CLA-1 L carries out its diverse functions.
活性区基因编码三种主要的异构体。长异构体CLA-1 L的功能不仅限于调节突触小泡胞吐作用，还包括突触小泡聚集和一种跨膜自噬蛋白的内吞分选。CLA-1 L含有一个大的N端重复区域。序列分析表明，该区域富含酸性残基，并呈现出无序结构，其间穿插着螺旋片段。虽然对重复区域的一部分进行建模揭示了紧密和较不紧密构象之间的动态转变，但对整个区域进行建模则表明其主要为伸展结构。这项研究揭示了CLA-1 L是如何执行其多种功能的。
