Xing Chaofan, Li Yong, Chen Zhenxiang, Hu Qingyuan, Sun Jiayi, Chen Huanyu, Zou Qi, Li Yingying, Yu Fei, Wang Chao, Wang Panpan, Shen Xin
Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China.
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China.
Biology (Basel). 2025 Jul 25;14(8):940. doi: 10.3390/biology14080940.
Appendage autotomy frequently occurs during the cultivation of , which severely impacts its survival and economic benefits. To investigate the molecular mechanism underlying appendage regeneration in , this study presents a comparative transcriptome analysis on samples from different stages of appendage regeneration in individuals of the same family of . A total of 6460 differentially expressed genes (DEGs) were identified between the samples collected at 0 h post-autotomy (D0) and those collected at 18 h post-autotomy (D18h). Additionally, 7740 DEGs were identified between D0 and 14 d post-autotomy (D14d), with 3382 DEGs identified between D18h and D14d. Among them, differentially expressed genes such as , , , and are related to muscle growth or molting and may be involved in the regeneration process. qRT-PCR results revealed that was expressed at relatively high levels in the gonad and ventral nerve cord tissues and that the highest level of expression was detected in the regenerative basal tissue at 24 h post-autotomy. In situ hybridization results indicated strong signals of this gene in the cells at the wound site at 72 h post-autotomy. Following knockdown of , the expression levels of both and were significantly downregulated, and long-term interference with the gene resulted in a significantly slower appendage regeneration process compared to the control group. When the downstream transcription factor was knocked down, the two receptor genes and were downregulated, whereas was upregulated. After inhibiting the BMP signaling pathway, the degree of cell aggregation at the autotomy site in the experimental group was significantly lower than that in the control group, the wound healing rate was delayed, and the blastema regeneration time was prolonged from 5 d to 7 d. Collectively, these results indicate that the BMP signaling pathway plays a critical role in the early stages of appendage regeneration in . This study provides important theoretical insights for understanding limb regeneration in crustaceans.
附肢自割现象在……养殖过程中频繁发生，这严重影响了其存活率和经济效益。为了探究……附肢再生的分子机制，本研究对同一家族个体附肢再生不同阶段的样本进行了比较转录组分析。在自割后0小时（D0）采集的样本与自割后18小时（D18h）采集的样本之间，共鉴定出6460个差异表达基因（DEG）。此外，在D0和自割后14天（D14d）之间鉴定出7740个DEG，在D18h和D14d之间鉴定出3382个DEG。其中，诸如……等差异表达基因与肌肉生长或蜕皮有关，可能参与了再生过程。qRT-PCR结果显示，……在性腺和腹神经索组织中表达水平相对较高，且在自割后24小时在再生基部组织中检测到最高表达水平。原位杂交结果表明，自割后72小时该基因在伤口部位的细胞中有强烈信号。敲低……后，……和……的表达水平均显著下调，与对照组相比，长期干扰……基因导致附肢再生过程明显变慢。当敲低下游转录因子……时，两个受体基因……和……下调，而……上调。抑制BMP信号通路后，实验组自割部位的细胞聚集程度明显低于对照组，伤口愈合速度延迟，胚基再生时间从5天延长至7天。总体而言，这些结果表明BMP信号通路在……附肢再生的早期阶段起着关键作用。本研究为理解甲壳类动物肢体再生提供了重要的理论见解。
