Qin Yue, Wang Yue, Wo Chunxin, Wang Zhenmin, Yu Zilong, Huang Yuanxin, Wang Lin
Clinical Medicine College, Guizhou Medical University, Guiyang, Guizhou, China.
Department of Algology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
PLoS One. 2025 Sep 4;20(9):e0330592. doi: 10.1371/journal.pone.0330592. eCollection 2025.
In clinical practice, silver needle thermal conduction therapy has a definite effect on myofascial pain syndrome (MPS). However, there is a lack of objective evidence to evaluate the efficacy of this therapy. This study aimed to assess the effectiveness of silver needle thermal conduction therapy on MPS rats by objective therapeutic index.
MPS model was established by blunt strick combined with centrifugal running training. MPS rats were divided into model and treatment groups, with a synchronized control group. The model group received no treatment, whereas the treatment group underwent silver needle thermal conduction therapy. The T2 value and stiffness values were evaluated by magnetic resonance imaging and sound touch elastography. The ultrastructure of muscle mitochondria was examined using transmission electron microscopy, and the Silent mating type information regulation 2 homolog 3 (SIRT3) expression level was evaluated by western blotting.
T2 values and elastic modulus values in the treatment group were lower than those in the model group, and there was no difference between the treatment group and the control group. Mitochondrial damage was observed in the model group, and the degree of mitochondrial damage in the treatment group was less than that in the model group. SIRT3 expression in the treatment group was down-regulated compared with the normal group, but up-regulated compared with the model group.
The silver needle thermal conduction therapy demonstrates the ability to reduce muscle inflammation and stiffness and facilitate the repair of damaged muscle mitochondria.
在临床实践中,银质针导热疗法对肌筋膜疼痛综合征(MPS)有确切疗效。然而,缺乏客观证据来评估该疗法的疗效。本研究旨在通过客观治疗指标评估银质针导热疗法对MPS大鼠的有效性。
采用钝性打击结合离心跑步训练建立MPS模型。将MPS大鼠分为模型组和治疗组,并设同步对照组。模型组不接受治疗,而治疗组接受银质针导热疗法。通过磁共振成像和声触诊弹性成像评估T2值和硬度值。使用透射电子显微镜检查肌肉线粒体的超微结构,并通过蛋白质印迹法评估沉默信息调节因子2同源物3(SIRT3)的表达水平。
治疗组的T2值和弹性模量值低于模型组,且治疗组与对照组之间无差异。模型组观察到线粒体损伤,治疗组的线粒体损伤程度小于模型组。与正常组相比,治疗组SIRT3表达下调,但与模型组相比上调。
银质针导热疗法具有减轻肌肉炎症和僵硬并促进受损肌肉线粒体修复的能力。