Heiniger Erin K, Jiang Kevin P, Kumar Sujatha, Yager Paul
Department of Bioengineering, University of Washington, Seattle, WA 98105, USA.
Analyst. 2025 Sep 5. doi: 10.1039/d5an00496a.
Curable sexually transmitted infections (STIs) caused by the bacteria (CT) and (NG) are widespread globally. These infections are particularly dangerous for female patients, causing pelvic inflammatory disease, infertility, and increased risk of HIV acquisition. Vaginal self-swab sampling can improve access to STI screening but is still subject to treatment delays due to centralized processing. A low-cost point-of-care (POC) device capable of detecting these bacteria from a self- or clinician-collected vaginal swab could address this delay and allow for more timely treatment. In this work, vaginal swab materials from patients infected with CT or NG required a filtration step before lysis and loop-mediated isothermal amplification (LAMP) detection using a UbiNAAT device. We have shown a simple, low-cost sample preparation method that supports rapid DNA detection from NG and CT on our POC UbiNAAT platform.
由沙眼衣原体（CT）和淋病奈瑟菌（NG）引起的可治愈性传播感染在全球广泛存在。这些感染对女性患者尤其危险，会导致盆腔炎、不孕，并增加感染艾滋病毒的风险。阴道自我采样可以改善性传播感染筛查的可及性，但由于集中处理，仍会出现治疗延迟的情况。一种能够从自我采集或临床医生采集的阴道拭子中检测出这些细菌的低成本即时检测（POC）设备可以解决这一延迟问题，并实现更及时的治疗。在这项研究中，感染CT或NG的患者的阴道拭子样本在使用UbiNAAT设备进行裂解和环介导等温扩增（LAMP）检测之前需要进行过滤步骤。我们展示了一种简单、低成本的样本制备方法，该方法支持在我们的即时检测UbiNAAT平台上对NG和CT进行快速DNA检测。
