Hamaya K, Doi K, Tanaka T, Nishimoto A
Acta Med Okayama. 1985 Dec;39(6):453-62. doi: 10.18926/AMO/31509.
Glial fibrillary acidic protein (GFAP) was purified from human spinal cord and cerebral white matter. GFAP was localized by an immuno-peroxidase method in normal adult and fetal human brains, rat brains, and 152 central nervous system (CNS) tumors. GFAP was found in reactive and normal astrocytes, immature cells of fetal brain at the 18th to 21st gestational weeks, and normal rat astrocytes. This GFAP staining was quite specific for glial tumors, including astrocytomas, glioblastomas, astroblastomas, and ependymomas. GFAP-positive cells were also found in oligodendrogliomas and choroid plexus papillomas, and they were interpreted as being astroglial or ependymal differentiations. Stromal cells in cerebellar hemangioblastomas were negative. However, engulfed astrocytes were found at the periphery of such tumors and often adjacent to the proliferate blood vessels. In meningiomas, neurinomas, metastatic carcinomas, pituitary adenomas and other non-glial tumors, GFAP-positive cells were not identified.
胶质纤维酸性蛋白（GFAP）从人脊髓和脑白质中纯化得到。通过免疫过氧化物酶法在正常成人和胎儿脑、大鼠脑以及152例中枢神经系统（CNS）肿瘤中对GFAP进行定位。在反应性和正常星形胶质细胞、妊娠第18至21周胎儿脑的未成熟细胞以及正常大鼠星形胶质细胞中发现了GFAP。这种GFAP染色对胶质肿瘤具有高度特异性，包括星形细胞瘤、胶质母细胞瘤、成星形细胞瘤和室管膜瘤。在少突胶质细胞瘤和脉络丛乳头状瘤中也发现了GFAP阳性细胞，它们被解释为星形胶质细胞或室管膜细胞分化。小脑成血管细胞瘤中的基质细胞呈阴性。然而，在此类肿瘤的周边发现了被吞噬的星形胶质细胞，且常与增殖的血管相邻。在脑膜瘤、神经鞘瘤、转移性癌、垂体腺瘤和其他非胶质肿瘤中，未发现GFAP阳性细胞。
