Kudamba Ali, Bbosa Godfrey S, Lugaajju Allan, Wabinga Henry, Niyonzima Nixon, Ocan Moses, Damani Ali M, Kafeero Hussein M, Ssenku Jamilu E, Okurut Shaban A, Lubowa Muniiru, Walusansa Abdul, Sivasundaram S Sivagami, Shampath Shobana, Muwonge Haruna
Department of Medical Physiology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda.
Faculty of Health Sciences, Habib Medical School, Islamic University in Uganda, Kampala, Uganda.
Med Oncol. 2025 Sep 5;42(10):463. doi: 10.1007/s12032-025-02953-5.
This study investigates the cytotoxic effects of R. tridentata extracts on prostate cancer cells, providing insight into its potential therapeutic benefits and scientific validation for its traditional use in cancer treatment. The cytotoxicity of R. tridentata extracts was evaluated on prostate cancer cell lines (LNCaP and DU 145) using the MTT assay, with doxorubicin as a reference standard. Our findings demonstrated significant concentration- and time-dependent cytotoxic effects of the extracts on both cell lines (p < 0.0024 to p < 0.0002). Notably, the methanol extract exhibited potent cytotoxicity, with IC values ranging from 124.07 to 211 μg/mL (DU145) and 100 to 180 μg/ml (LNCaP) over 24-72 h (p < 0.0024 to p < 0.0001), highlighting its therapeutic potential. This study unveils the promising cytotoxic activity of R. tridentata extracts, particularly methanol extracts, against prostate cancer cells, showcasing their concentration- and time-dependent effects. These findings underscore the potential of plant-derived extracts as therapeutic agents for cancer treatment, warranting further research.
本研究调查了三叉苦提取物对前列腺癌细胞的细胞毒性作用，深入了解其潜在的治疗益处，并为其在癌症治疗中的传统应用提供科学验证。以阿霉素作为参考标准，采用MTT法评估了三叉苦提取物对前列腺癌细胞系（LNCaP和DU 145）的细胞毒性。我们的研究结果表明，提取物对两种细胞系均具有显著的浓度和时间依赖性细胞毒性作用（p < 0.0024至p < 0.0002）。值得注意的是，甲醇提取物表现出强大的细胞毒性，在24至72小时内，其IC值范围为124.07至211μg/mL（DU145）和100至180μg/ml（LNCaP）（p < 0.0024至p < 0.0001），突出了其治疗潜力。本研究揭示了三叉苦提取物，特别是甲醇提取物，对前列腺癌细胞具有有前景的细胞毒性活性，展示了其浓度和时间依赖性作用。这些发现强调了植物提取物作为癌症治疗药物的潜力，值得进一步研究。
