Topp H, Sander G, Heller-Schöch G, Schöch G
Anal Biochem. 1985 Nov 1;150(2):353-8. doi: 10.1016/0003-2697(85)90522-6.
A simple and reliable HPLC method for quantitative determination of pseudouridine and uric acid in human urine and serum using a cation-exchange resin is described. This method is straightforward (12 runs of urine samples per day since the sample is only diluted into buffer and then chromatographed), sensitive, and highly reproducible. The column is stable over long periods (approximately 3 months of uninterrupted use at a time; it is thereafter easily restored to the original state). Mean excretion values for pseudouridine (in mumol/mmol creatinine) are 26.4 +/- 3.1 (17 female adults), 23.8 +/- 2.5 (12 male adults), 164.7 +/- 32.2 (37 male preterm infants); mean values for uric acid (mumol/mmol creatinine) are, respectively, 310.3 +/- 90.5, 278.2 +/- 56.1, and 1108 +/- 314. Human serum is deproteinized by pressure ultrafiltration in microcollodion bags with a nominal exclusion molecular weight of 12,400 and then put directly onto the HPLC column. The complete procedure takes 4 h.
描述了一种使用阳离子交换树脂定量测定人尿液和血清中假尿苷和尿酸的简单可靠的高效液相色谱法。该方法简单直接（由于样品只需稀释到缓冲液中然后进行色谱分析，每天可运行12次尿液样本）、灵敏且重现性高。该色谱柱长期稳定（一次可连续使用约3个月；之后可轻松恢复到原始状态）。假尿苷的平均排泄值（以μmol/mmol肌酐计）为26.4±3.1（17名成年女性）、23.8±2.5（12名成年男性）、164.7±32.2（37名男性早产儿）；尿酸的平均值（μmol/mmol肌酐）分别为310.3±90.5、278.2±56.1和1108±314。人血清通过在标称排阻分子量为12400的微胶棉袋中进行压力超滤进行脱蛋白，然后直接注入高效液相色谱柱。整个过程耗时4小时。
