Aberhart D J, Cotting J A, Lin H J
Anal Biochem. 1985 Nov 15;151(1):88-91. doi: 10.1016/0003-2697(85)90056-9.
For studies on the coenzyme B12-dependent enzyme, leucine-2,3-aminomutase, (3R)- and (3S)-beta-leucines were synthesized. The 10-camphorsulfonamide p-nitrobenzyl esters could be resolved by normal-phase HPLC. A much better separation was obtained by reversed-phase HPLC of the diastereomeric derivatives obtained by treatment of -leucine with Marfey's reagent (N2-(5-fluoro-2,4-dinitrophenyl)-L-alaninamide).
为了研究依赖辅酶B12的酶——亮氨酸-2,3-氨基变位酶，合成了(3R)-和(3S)-β-亮氨酸。10-樟脑磺酰胺对硝基苄酯可通过正相高效液相色谱法拆分。用马尔费试剂（N2-(5-氟-2,4-二硝基苯基)-L-丙氨酰胺）处理β-亮氨酸得到的非对映体衍生物，通过反相高效液相色谱法可实现更好的分离。
