通过高效液相色谱法分离α-和β-氨基酸：应用于赖氨酸2,3-氨基变位酶和亮氨酸2,3-氨基变位酶的测定
Separation by high-performance liquid chromatography of alpha- and beta-amino acids: application to assays of lysine 2,3-aminomutase and leucine 2,3-aminomutase.
作者信息
Aberhart D J
机构信息
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
出版信息
Anal Biochem. 1988 Mar;169(2):350-5. doi: 10.1016/0003-2697(88)90294-1.
Several pairs of alpha- and beta-amino acids were well separated as the ortho-phthalaldehyde derivatives by reversed phase HPLC. These included alpha- and beta-lysine, leucine, tyrosine, serine, aminoisobutyric acid, and beta-hydroxyvaline and its positional isomer. The separation was applicable to assays of lysine 2,3-aminomutase and leucine 2,3-aminomutase. However, for unknown reasons, no leucine 2,3-aminomutase activity could be found in rat liver homogenate.
通过反相高效液相色谱法，几对α-氨基酸和β-氨基酸作为邻苯二甲醛衍生物得到了很好的分离。这些氨基酸包括α-赖氨酸、β-赖氨酸、亮氨酸、酪氨酸、丝氨酸、氨基异丁酸、β-羟基缬氨酸及其位置异构体。该分离方法适用于赖氨酸2,3-氨基变位酶和亮氨酸2,3-氨基变位酶的测定。然而，不知为何，在大鼠肝脏匀浆中未发现亮氨酸2,3-氨基变位酶活性。
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