Peck W A, Rifas L, Shen V
Ann Biol Clin (Paris). 1985;43(5):751-4.
Cells of the monocyte-macrophage series play multiple roles in bone resorption; they appear to act as osteoclast precursors and are known to elaborate several substances which promote osteoclast activity. Because bone formation is closely linked (coupled) to resorption under physiological conditions, we explored the possibility that macrophages also elaborate a stimulator of bone formation. Our results indicate that rat resident peritoneal and bone marrow macrophages elaborate a potent stimulator of DNA synthesis and growth in osteoblasts (as well as chondrocytes) cultured from the calvaria of rat fetuses. Growth factor activity resides mainly in a 43 000-dalton heat-stable protein, though a lower molecular weight peptide (Mr approximately 10 000) also contained growth-promoting activity. In contrast to osteoblasts and chondrocytes, skin fibroblasts prepared from the same rat fetuses exhibited only a minimal response to the macrophage-derived factor, though they responded briskly to epidermal and fibroblast growth factors. These findings are consistent with the thesis that macrophages direct both phases of remodeling, resorption and formation, and therefore may be responsible for coupling these events.
单核细胞-巨噬细胞系列的细胞在骨吸收中发挥多种作用；它们似乎作为破骨细胞前体起作用，并且已知能分泌几种促进破骨细胞活性的物质。由于在生理条件下骨形成与吸收密切相关（耦合），我们探讨了巨噬细胞是否也能分泌一种骨形成刺激物的可能性。我们的结果表明，大鼠腹腔常驻巨噬细胞和骨髓巨噬细胞能分泌一种对从大鼠胎儿颅骨培养的成骨细胞（以及软骨细胞）的DNA合成和生长有强大刺激作用的物质。生长因子活性主要存在于一种43000道尔顿的热稳定蛋白中，不过一种分子量较低的肽（约10000道尔顿）也含有促进生长的活性。与成骨细胞和软骨细胞不同，从相同大鼠胎儿制备的皮肤成纤维细胞对巨噬细胞衍生因子仅表现出最小的反应，尽管它们对表皮生长因子和成纤维细胞生长因子反应迅速。这些发现与巨噬细胞指导重塑的两个阶段（吸收和形成）的观点一致，因此可能负责将这些事件耦合起来。
