Han Ji Yeon, Kim Yoon Young, Choi Bo Bin, Kim Sung Woo, Ku Seung-Yup
Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, 03080, Korea.
Tissue Eng Regen Med. 2025 Sep 6. doi: 10.1007/s13770-025-00761-6.
Endometrial damage is a critical factor contributing to infertility, particularly in women with refractory thin endometrium or intrauterine adhesions. Therefore, developing innovative therapeutic strategies for endometrial regeneration is essential. This study evaluates the regenerative potential of endometrial stromal cell (EMSC) injection and EMSC-loaded patch application in a mouse model with ethanol-induced endometrial damage.
A mouse model of endometrial damage was established using ethanol injection into the uterine horn. EMSCs were isolated, cultured, and either HA-injected into the damaged endometrium or transplanted via a small intestinal submucosa (SIS)-based EMSC patch. Histological analyses were performed to assess endometrial thickness, gland regeneration, and fibrosis reduction.
Both EMSC injection and SIS-based EMSC patch engraftment promoted endometrial regeneration. However, the SIS-based EMSC patch group exhibited significant improvements in endometrial thickness, gland formation, and fibrosis reduction compared to the EMSC injection group.
This study demonstrates the superior regenerative potential of an SIS-based EMSC patch over direct EMSC injection for endometrial repair. The findings suggest that scaffold-assisted cell therapy could be a promising approach for treating endometrial damage-related infertility. Further studies are required to optimize this strategy for clinical applications.
子宫内膜损伤是导致不孕的关键因素，尤其是对于难治性薄型子宫内膜或宫腔粘连的女性。因此，开发创新的子宫内膜再生治疗策略至关重要。本研究评估了在乙醇诱导的子宫内膜损伤小鼠模型中，注射子宫内膜基质细胞（EMSC）和应用负载EMSC的贴片的再生潜力。
通过向子宫角注射乙醇建立子宫内膜损伤小鼠模型。分离、培养EMSC，然后将其注射到受损的子宫内膜中，或通过基于小肠黏膜下层（SIS）的EMSC贴片进行移植。进行组织学分析以评估子宫内膜厚度、腺体再生和纤维化减轻情况。
EMSC注射和基于SIS的EMSC贴片植入均促进了子宫内膜再生。然而，与EMSC注射组相比，基于SIS的EMSC贴片组在子宫内膜厚度、腺体形成和纤维化减轻方面表现出显著改善。
本研究表明，对于子宫内膜修复，基于SIS的EMSC贴片比直接注射EMSC具有更强的再生潜力。研究结果表明，支架辅助细胞疗法可能是治疗子宫内膜损伤相关不孕症的一种有前景的方法。需要进一步研究以优化该策略用于临床应用。
