Long Li, Jia Maohang, Feng Shuang, Long Zhangfu, Xu Heng
Key Laboratory of Bio-resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, China.
Key Laboratory of Bio-resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, China; Key Laboratory of Environment Protection, Soil ecological protection and pollution control, Sichuan University & Department of Ecology and Environment of Sichuan, Chengdu, 610065, Sichuan, China.
Fungal Biol. 2025 Oct;129(6):101633. doi: 10.1016/j.funbio.2025.101633. Epub 2025 Jul 28.
Cadmium (Cd) contamination in edible fungi poses a significant threat to food safety. However, targeted strategies to regulate Cd uptake and enhance Cd stress tolerance in Morchella sextelata remain largely unexplored. Given that M. sextelata mycelia can autonomously adsorb beneficial metal ions to promote growth, regulating Cd absorption through ion-ion interactions emerges as a promising approach. In this study, under 1 mg/L Cd stress, the exogenous application of Fe and Mn at a 1:1 M ratio significantly increased mycelial biomass by 20.49 % and 22.11 %, respectively, and effectively reduced Cd accumulation. In contrast, Mg led to a moderate biomass increase of 8.94 %. Notably, Fe effectively inhibited Cd accumulation in mycelia, reducing Cd content by 81.76 %. Moreover, the addition of divalent ions significantly alleviated osmotic stress in the mycelia, preventing the efflux of sugars and proteins. LC-MS/MS-based metabolomic profiling identified 1446 metabolites. One-way ANOVA revealed distinct metabolic changes associated with metal ion treatments under Cd stress. KEGG pathway enrichment indicated that histidine metabolism plays a key role in the Cd stress mitigation process. VIP (Variable Importance in Projection) analysis further identified key metabolites involved in the repair response. Correlation analysis highlighted carnitine as a core metabolite significantly associated with phenotypic improvements across all treatments. In summary, this study presents the first attempt to regulate Cd accumulation in M. sextelata through divalent ion application. It provides novel insights into the physiological and metabolic mechanisms underlying this ion-mediated mitigation strategy.
食用菌中的镉（Cd）污染对食品安全构成重大威胁。然而，调控六棱羊肚菌对镉的吸收并增强其对镉胁迫耐受性的针对性策略在很大程度上仍未得到探索。鉴于六棱羊肚菌菌丝体能自主吸附有益金属离子以促进生长，通过离子-离子相互作用调控镉吸收成为一种有前景的方法。在本研究中，在1 mg/L镉胁迫下，以1:1摩尔比外源施加铁和锰分别使菌丝体生物量显著增加了20.49%和22.11%，并有效降低了镉积累。相比之下，镁使生物量适度增加了8.94%。值得注意的是，铁有效抑制了菌丝体中的镉积累，使镉含量降低了81.76%。此外，二价离子的添加显著缓解了菌丝体中的渗透胁迫，防止了糖类和蛋白质的外流。基于液相色谱-串联质谱的代谢组学分析鉴定出1446种代谢物。单因素方差分析揭示了镉胁迫下与金属离子处理相关的明显代谢变化。KEGG通路富集表明组氨酸代谢在缓解镉胁迫过程中起关键作用。投影变量重要性（VIP）分析进一步确定了参与修复反应的关键代谢物。相关性分析突出了肉碱是与所有处理中表型改善显著相关的核心代谢物。总之，本研究首次尝试通过施加二价离子来调控六棱羊肚菌中的镉积累。它为这种离子介导的缓解策略背后的生理和代谢机制提供了新的见解。
