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[突尼斯单峰驼(骆驼科,哺乳纲)下丘脑 - 垂体后叶系统肽类激素的研究]

[Study of the peptide hormones of the hypothalamo-postpituitary system of the dromedary, Camelus dromedarius, (Camelidae, Mammalia) of Tunisia].

作者信息

Marrakchi-Mzid N, Morel A, Camier M, Djegham M, Cohen A, Belhadj N

出版信息

Arch Inst Pasteur Tunis. 1985 Sep;62(3):329-38.

PMID:4091625
Abstract

Ocytocin and vasopressin are two nonapeptides of the hypo thalamo-Post-hypophysary system. They are isolated and purified from dromedary post-hyphophysis, freshly collected in the meat centers of the south of Tunisia. The use of three chromatographies steps were found to be essential in obtaining high Pure hormones. These steps cousist in a sephadex G25 gel filtration and two successive High performance liquid chromatographies: H.P.L.C. The analysis of the two hormones by thin layer chromatography and Radioimmunoassays, and the identification of the amino-acid composition demonstrate that camel vasopressin is a 8 arginin vasopressin but ocytocin is identic to the other hormones isolated from different animal species.

摘要

催产素和加压素是下丘脑 - 垂体后叶系统的两种九肽。它们是从突尼斯南部肉类中心新鲜采集的单峰驼垂体后叶中分离和纯化出来的。发现使用三个色谱步骤对于获得高纯度激素至关重要。这些步骤包括葡聚糖凝胶G25凝胶过滤和两次连续的高效液相色谱法:H.P.L.C.。通过薄层色谱法和放射免疫测定法对这两种激素进行分析,以及对氨基酸组成的鉴定表明,骆驼加压素是8 - 精氨酸加压素,但催产素与从不同动物物种中分离出的其他激素相同。

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