Gruber K A, Stein S, Brink L, Radhakrishnan A, Udenfriend S
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1314-8. doi: 10.1073/pnas.73.4.1314.
A fluorometric method for the quantitative assay of vasopressin and oxytocin in individual rat pituitaries has been developed. Acid extracts of pituitaries are freed of amino acids and polyamines by passage over a copper-Sephadex column, and the peptides fraction is then labeled by reaction with fluorescamine. The resulting peptide fluorophors are separated by chromatography on a reverse-phase bonded column. Specificity of the procedure was ascertained by several criteria, including bioassay and amino-acid analysis of the eluted peptide fluorophors. The procedure serves as a model system for the assay of tissue peptides in the picomole range.
已开发出一种用于定量测定单个大鼠垂体中血管加压素和催产素的荧光测定法。垂体的酸性提取物通过铜-葡聚糖凝胶柱去除氨基酸和多胺,然后肽段通过与荧光胺反应进行标记。所得的肽荧光团通过反相键合柱色谱法分离。该方法的特异性通过多种标准确定,包括对洗脱的肽荧光团进行生物测定和氨基酸分析。该方法可作为皮摩尔范围内组织肽测定的模型系统。