Ficoseco Cecilia M Aristimuño, Chieffi Daniele, Montemurro Marco, Bavaro Annarita, Rizzello Carlo G, Nader-Macias Maria E F, Fadda Silvina, Fanelli Francesca, Fusco Vincenzina, Vignolo Graciela M
Reference Center for Lactobacilli (CERELA-CONICET), San Miguel de Tucumán, Argentina.
National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, Italy.
Environ Microbiol Rep. 2025 Oct;17(5):e70176. doi: 10.1111/1758-2229.70176.
Limosilactobacillus fermentum CRL2085, isolated from feedlot cattle rations, displayed high efficiency as a probiotic when administered to animals. A comprehensive genomic analysis was performed to elucidate the genetic basis underlying its probiotic potential. Fifteen genomic islands and CRISPR-Cas elements were identified in its genome. Pan-genomic analysis highlighted the dynamic evolution of this species, and clustering based on the nucleotide genomic similarity only partially correlated with the source of isolation or the geographic origin of the strains. Several genes known to confer probiotic properties were identified, including those related to adhesion, resistance to acidic pH and bile salts, tolerance to oxidative stress, metabolism/transport of sugars and other compounds, and genes for exopolysaccharide biosynthesis. In silico analysis of antimicrobial resistance genes and virulence determinants confirmed the safety of this strain. Moreover, genes related to B-group vitamins biosynthesis and feruloyl esterase hydrolase were also found, showing the nutritional contribution of the strain, which also showed moderate adhesion capability, exopolysaccharide production when grown with sucrose, and the capacity to metabolise 42 out of 95 carbon substrates tested. This data provides the genetic basis for deciphering the mechanisms beyond the benefits demonstrated by its use during cattle intensive raising and confirms its promising role as a probiotic.
从饲养场牛日粮中分离出的发酵乳杆菌属弯曲乳杆菌CRL2085，在给动物投喂时作为益生菌表现出高效性。进行了全面的基因组分析，以阐明其益生菌潜力背后的遗传基础。在其基因组中鉴定出15个基因组岛和CRISPR-Cas元件。泛基因组分析突出了该物种的动态进化，基于核苷酸基因组相似性的聚类仅部分与菌株的分离来源或地理起源相关。鉴定出了几个已知赋予益生菌特性的基因，包括那些与粘附、耐酸性pH值和胆盐、耐氧化应激、糖和其他化合物的代谢/转运以及胞外多糖生物合成相关的基因。对抗菌素耐药基因和毒力决定因素的电子分析证实了该菌株的安全性。此外，还发现了与B族维生素生物合成和阿魏酸酯酶水解酶相关的基因，显示了该菌株的营养贡献，该菌株还表现出适度的粘附能力，在与蔗糖一起生长时产生胞外多糖，以及能够代谢所测试的95种碳底物中的42种。这些数据为解读其在牛集约化饲养期间所展现益处背后的机制提供了遗传基础，并证实了其作为益生菌的前景广阔。
