Zhang Qi, Li Jiahao, Zhang Yuqing, Zhang Hongsheng, Wang Cong, Ma Qiang
Chinese Academy of Quality and Inspection & Testing Beijing China.
NMPA Key Laboratory for POCT Technology Transforming and Quality Control Beijing China.
Anal Sci Adv. 2025 Sep 6;6(2):e70041. doi: 10.1002/ansa.70041. eCollection 2025 Dec.
Single-cell analysis provides critical insights into cellular heterogeneity, dynamic behaviours and microenvironmental interactions, driving advancements in precision medicine and disease mechanism research. However, traditional technologies face limitations due to low throughput, insufficient sensitivity and bottlenecks in multi-omics integration. Microdroplet printing technology, with its advantages in high-throughput single-cell encapsulation, picolitre-level reaction precision and oil-free phase contamination avoidance, has propelled single-cell analysis into a new era of high-throughput and high-dimensional resolution through deep integration with multimodal detection platforms. This review systematically elaborates on the theoretical framework, diverse technical systems and multi-dimensional application scenarios of microdroplet printing technology. It further dissects the deep coupling mechanisms between this technology and multimodal detection platforms such as including mass spectrometry, Raman spectroscopy, fluorescence and ultraviolet detectors, as well as its unique advantages in single-cell analysis. Such cross-technology integration has significantly accelerated innovation in fields such as single-cell drug screening and multi-omics analysis, marking a significant leap in the evolution of single-cell analytical methodologies.
单细胞分析为细胞异质性、动态行为和微环境相互作用提供了关键见解，推动了精准医学和疾病机制研究的进展。然而，传统技术由于通量低、灵敏度不足以及多组学整合的瓶颈而面临局限性。微滴打印技术在高通量单细胞封装、皮升级反应精度和避免无油相污染方面具有优势，通过与多模态检测平台深度整合，将单细胞分析推进到了高通量和高维分辨率的新时代。本文综述系统阐述了微滴打印技术的理论框架、多样的技术体系和多维应用场景。它进一步剖析了该技术与质谱、拉曼光谱、荧光和紫外探测器等多模态检测平台之间的深度耦合机制，以及其在单细胞分析中的独特优势。这种跨技术整合显著加速了单细胞药物筛选和多组学分析等领域的创新，标志着单细胞分析方法学发展的重大飞跃。
