Vilím V
Biomed Biochim Acta. 1985;44(11-12):1717-20.
Conditions allowing substitution of 3-hydroxydiphenyl, recommended by N. Blumenkrantz and G. Asboe-Hansen (Anal. Biochem. 54, 484-489, 1973) as a reagent for estimation of hexuronic acids by the much cheaper 2-hydroxydiphenyl were investigated. The absorption maximum of a color product formed by glucuronic acid and 2-hydroxydiphenyl was found to be at 475 nm. Using this wavelength for measurement, and slightly modified reaction conditions, the sensitivity achieved by using both reagents was found to be comparable. The only interference was that of DNA, encountered in nucleic acid-rich unpurified soft tissues extracts.
研究了由N. 布卢门克兰茨和G. 阿斯博 - 汉森（《分析生物化学》54卷，484 - 489页，1973年）推荐的，用便宜得多的2 - 羟基二苯基替代3 - 羟基二苯基作为测定己糖醛酸试剂的条件。发现葡萄糖醛酸与2 - 羟基二苯基形成的有色产物的最大吸收波长在475 nm。使用该波长进行测量，并对反应条件稍作修改后，发现使用这两种试剂所达到的灵敏度相当。唯一的干扰是在富含核酸的未纯化软组织提取物中遇到的DNA干扰。
