Schneider Cassidy, Koury Spencer, Stevison Laurie S
Biological Sciences, Auburn University, Auburn, Alabama, United States.
MicroPubl Biol. 2025 Aug 21;2025. doi: 10.17912/micropub.biology.001656. eCollection 2025.
Measures of organismal fitness must take into account reproductive output and survivorship across life-history stages. In , a laboratory model system, these traits are often quantified with egg-laying assays and egg-to-adult viability. While several protocols for automated egg counting exist, these methods typically preclude directly analyzing phenotypic distributions in resulting adults. Here, we digitally score eggs from photographs taken under standard laboratory culturing conditions (cornmeal media in polystyrene vials) using a method compatible with scoring surviving adults. This absolute measurement of egg-to-adult viability, that also allows investigation of survivorship biases, was applied to three commonly used laboratory strains.
衡量生物体适应性的指标必须考虑到整个生命史阶段的繁殖产出和存活率。在实验室模型系统中，这些特征通常通过产卵测定和卵到成虫的存活率来量化。虽然存在几种自动计数卵的方案，但这些方法通常排除了直接分析所产生成虫的表型分布。在这里，我们使用一种与对存活成虫评分兼容的方法，对在标准实验室培养条件下（聚苯乙烯小瓶中的玉米粉培养基）拍摄的照片中的卵进行数字评分。这种对卵到成虫存活率的绝对测量，也允许对存活偏差进行研究，已应用于三种常用的实验室菌株。
