Shlesinger H, Ovil Y, Levy M J, Kessler-Icekson G
Biochem Int. 1985 Nov;11(5):747-53.
A discrepancy observed in electrophoretic mobility of human atrial and ventricular myosin heavy chains permitted the determination of these protein isoforms in myocardial biopsies. In ventricular biopsies we found only the heavy chain specific to this tissue whereas in atrial specimens we sometimes detected an extra heavy chain comigrating with the ventricular species. The peptide mapping of such preparations revealed peptides unique to ventricular heavy chain. Patients suffering from pressure overload expressed ventricular heavy chain at a higher frequency than those suffering from coronary heart disease (66% VS.29%), but this disparity was not found statistically significant (0.1 greater than P greater than 0.05).
人类心房和心室肌球蛋白重链在电泳迁移率上的差异，使得在心肌活检中能够确定这些蛋白质异构体。在心室活检中，我们仅发现了该组织特有的重链，而在心房标本中，我们有时会检测到一条与心室重链共同迁移的额外重链。此类制剂的肽图谱显示了心室重链特有的肽段。压力超负荷患者表达心室重链的频率高于冠心病患者（66% 对 29%），但这种差异在统计学上无显著意义（0.1 > P > 0.05）。
