Srihari T, Tuchschmid C R, Schaub M C
Basic Res Cardiol. 1982 Nov-Dec;77(6):599-609. doi: 10.1007/BF01908313.
The light chains of myosin from atrial and ventricular tissues from rat and rabbit were examined by one- and two-dimensional polyacrylamide gel electrophoresis. The myosin heavy chains were electrophoretically isolated, digested after denaturation in sodium dodecyl sulfate with papain and proteinase from S. aureus V8, and the resulting peptides resolved in one-dimensional gel electrophoresis. The peptide patterns of myosin heavy chains from atrial and ventricular tissues of adult rabbits were different, indicating differences in their primary structures. No such differences could be detected in a total of around 180 peptides produced by the two proteinases from the myosin heavy chains of adult rat atrial and ventricular tissues. With regard to light chains, the same migration pattern was observed for atrial and ventricular tissues from both rat and rabbit. The atrial light chains ALC1 and ALC2 migrated with molecular weights lying between those of the ventricular light chains VLC1 and VLC2. In two-dimensional electrophoresis, the corresponding light chains from rat and rabbit co-migrated. An additional light chain was observed in foetal ventricles, which exhibited identical electrophoretic properties to ALC1 from adult atrial tissues. In rat myofibrillar preparations from atrium and ventricle, an unidentified protein (x) occurred in the region of light chain-1 but with a more acidic isoelectric point, which seems to be related to the developmental stage of these tissues and which could not be detected in rabbit heart tissues or in any skeletal muscles.
采用一维和二维聚丙烯酰胺凝胶电泳法，对大鼠和家兔心房及心室组织中的肌球蛋白轻链进行了检测。对肌球蛋白重链进行电泳分离，在十二烷基硫酸钠中变性后，用木瓜蛋白酶和金黄色葡萄球菌V8蛋白酶进行消化，然后将所得肽段在一维凝胶电泳中进行分离。成年家兔心房和心室组织中肌球蛋白重链的肽段图谱不同，表明其一级结构存在差异。成年大鼠心房和心室组织的肌球蛋白重链经两种蛋白酶作用产生的总共约180个肽段中，未检测到此类差异。关于轻链，大鼠和家兔的心房和心室组织观察到相同的迁移模式。心房轻链ALC1和ALC2的迁移分子量介于心室轻链VLC1和VLC2之间。在二维电泳中，大鼠和家兔相应的轻链共同迁移。在胎儿心室中观察到一条额外的轻链，其电泳性质与成年心房组织的ALC1相同。在大鼠心房和心室的肌原纤维制剂中，在轻链-1区域出现一种未鉴定的蛋白质（x），但其等电点更偏酸性，这似乎与这些组织发育阶段有关，在家兔心脏组织或任何骨骼肌中均未检测到。
