Steensgaard J, Jacobsen C
J Immunol Methods. 1979;29(2):173-83. doi: 10.1016/0022-1759(79)90067-x.
A new gradient former for production of optimized sucrose gradients for B-XIV zonal rotors is described. Optimized gradients are gradients with an initial shelf to provide capacity and a shape that ensures isokinetic sedimentation conditions. By exhaustive computer calculations it has been found that optimized gradients can be produced by a very simple two-compartment gradient former. The design is given. Moreover, graphs for choice of gradient strength and run duration for preparative and analytical runs of samples containing soluble immune complexes are calculated. The new gradient former allows the use of a very simple procedure for zonal centrifugation.
本文描述了一种用于为B-XIV区带转子生产优化蔗糖梯度的新型梯度形成器。优化梯度是指具有初始平台以提供容量且形状能确保等速沉降条件的梯度。通过详尽的计算机计算发现，非常简单的双隔室梯度形成器就能产生优化梯度。文中给出了该设计。此外，还计算了用于含有可溶性免疫复合物样品的制备性和分析性运行的梯度强度和运行持续时间选择的图表。这种新型梯度形成器允许采用非常简单的区带离心程序。
