Bagwe Parab Siddhi, Kaur Ginpreet
Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, SVKM's Narsee Monjee Institute of Management Studies, Mumbai, 56, India.
Department of Pharmacology, SVKM's Dr. Bhanuben Nanavati College of Pharmacy, V. M. Road, Vile Parle (W), Mumbai, 56, India.
Fish Physiol Biochem. 2025 Sep 8;51(5):160. doi: 10.1007/s10695-025-01575-y.
Zebrafish models have been used to research Alzheimer's disease and other neurodegenerative disorders because of their similarities to the human genetic composition and behavior. Researchers have detected iron accumulation in the post-mortem brain sections of neurodegenerative disorder patients. Therefore, the development an animal model to simulate these clinical pathological findings is important. Iron is an important metal for maintaining homeostasis in the brain, depletion and accumulation of iron hamper neuronal development. Given the importance of iron overload in cognition impairment, this research aimed to develop an iron-induced zebrafish model of cognitive impairment. Zebrafish were subjected to ferrous sulfate (1.5 mg/L, 3 mg/L, and 6 mg/L) for 28 days. The behavioral parameters (Y-maze, novel tank test), oxidative stress parameters (MDA, GSH, and catalase), acetylcholinesterase (AChE) levels, iron levels, and interleukin-1β (IL-1β) levels in brain homogenate were assessed. The behavioral and locomotor responses, specifically in the zebrafish treated with iron for 28 days, suggest an increase in the loss of spatial memory and anxiety. Reactive oxygen species in the brain significantly increased (p < 0.001) with the increase in concentrations of iron. Brain tissue iron content and IL-1β significantly increased (p < 0.001) in the brain homogenate of the zebrafish. This model will aid in the screening of therapeutic compounds to accelerate drug discovery in the field of neurodegenerative diseases.
由于斑马鱼的基因组成和行为与人类相似，因此已被用于研究阿尔茨海默病和其他神经退行性疾病。研究人员在神经退行性疾病患者的死后脑切片中检测到铁积累。因此，开发一种动物模型来模拟这些临床病理发现很重要。铁是维持大脑内稳态的重要金属，铁的消耗和积累会阻碍神经元发育。鉴于铁过载在认知障碍中的重要性，本研究旨在建立一种铁诱导的斑马鱼认知障碍模型。将斑马鱼暴露于硫酸亚铁（1.5毫克/升、3毫克/升和6毫克/升）中28天。评估了行为参数（Y迷宫、新水箱试验）、氧化应激参数（丙二醛、谷胱甘肽和过氧化氢酶）、乙酰胆碱酯酶（AChE）水平、铁水平和脑匀浆中的白细胞介素-1β（IL-1β）水平。行为和运动反应，特别是在经铁处理28天的斑马鱼中，表明空间记忆丧失和焦虑增加。随着铁浓度的增加，大脑中的活性氧显著增加（p<0.001）。斑马鱼脑匀浆中的脑组织铁含量和IL-1β显著增加（p<0.001）。该模型将有助于筛选治疗性化合物，以加速神经退行性疾病领域的药物发现。
