One of the most prevalent metabolic diseases in recent years, type 2 diabetes is now one of the top causes of death globally and a significant risk factor for cardiovascular diseases. Therefore, the goal of this study is to investigate the impact of HIIT exercises on the levels of specific proteins associated with mitochondrial biogenesis and apoptosis in the heart tissue of male Wistar rats with type 2 diabetes. Animals in diabetic groups were given a high-fat diet and an intraperitoneal injection of STZ to cause diabetes. Following confirmation of the induction of diabetes, the exercise groups engaged in an 8-week HIIT exercise program. The current study's findings show that in the heart tissue of diabetic rats, the creation of diabetes significantly raised P53 protein levels and significantly decreased SIRT1 protein levels. On the other hand, HIIT decreased P53 expression and raised SIRT1.